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. 1975 Jun;122(3):826–831. doi: 10.1128/jb.122.3.826-831.1975

Assembly of the mitochondrial membrane system: isolation of nuclear and cytoplasmic mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions.

A Tzagoloff, A Akai, R B Needleman
PMCID: PMC246131  PMID: 168180

Abstract

A selection procedure is described which permits a large number of Saccharomyces cerevisiae mutants to be screened for specific lesions in mitochondrial respiratory enzymes and the adenosine triphosphatase. The method has been used to isolate nuclear mutant strains with specific lesions in coenzyme QH2-cytochrome c reductase, cytochrome oxidase, and adenosine triphosphatase. In addition, two cytoplasmic mutants have been found whose primary defect is in cytochrome oxidase, and others have been found that show variable degrees of abnormalities in their mitochondrial translation products.

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Selected References

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  1. EPHRUSSI B., SLONIMSKI P. P., PERRODIN G. La synthèse adaptative des cytochromes chez la levure de boulangerie. Biochim Biophys Acta. 1950 Nov;6(2):256–267. doi: 10.1016/0006-3002(50)90098-9. [DOI] [PubMed] [Google Scholar]
  2. Ebner E., Mennucci L., Schatz G. Mitochondrial assembly in respiration-deficient mutants of Saccharomyces cerevisiae. I. Effect of nuclear mutations on mitochondrial protein synthesis. J Biol Chem. 1973 Aug 10;248(15):5360–5368. [PubMed] [Google Scholar]
  3. Flury U., Mahler H. R., Feldman F. A novel respiration-deficient mutant of Saccharomyces cerevisiae. I. Preliminary characterization of phenotype and mitochondrial inheritance. J Biol Chem. 1974 Oct 10;249(19):6130–6137. [PubMed] [Google Scholar]
  4. Goldring E. S., Grossman L. I., Krupnick D., Cryer D. R., Marmur J. The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide. J Mol Biol. 1970 Sep 14;52(2):323–335. doi: 10.1016/0022-2836(70)90033-1. [DOI] [PubMed] [Google Scholar]
  5. Kuzela S., Grecná E. Lack of amino acid incorporation by isolated mitochondria from respiratory-deficient cytoplasmic yeast mutants. Experientia. 1969;25(7):776–777. doi: 10.1007/BF01897625. [DOI] [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Mason T. L., Schatz G. Cytochrome c oxidase from bakers' yeast. II. Site of translation of the protein components. J Biol Chem. 1973 Feb 25;248(4):1355–1360. [PubMed] [Google Scholar]
  8. Putrament A., Baranowska H., Prazmo W. Induction by manganese of mitochondrial antibiotic resistance mutations in yeast. Mol Gen Genet. 1973 Nov 22;126(4):357–366. doi: 10.1007/BF00269445. [DOI] [PubMed] [Google Scholar]
  9. Rubin M. S., Tzagoloff A. Assembly of the mitochondrial membrane system. X. Mitochondrial synthesis of three of the subunit proteins of yeast cytochrome oxidase. J Biol Chem. 1973 Jun 25;248(12):4275–4279. [PubMed] [Google Scholar]
  10. SHERMAN F., SLONIMSKI P. P. RESPIRATION-DEFICIENT MUTANTS OF YEAST. II. BIOCHEMISTRY. Biochim Biophys Acta. 1964 Jul 15;90:1–15. doi: 10.1016/0304-4165(64)90113-8. [DOI] [PubMed] [Google Scholar]
  11. Schatz G., Saltzgaber J. Protein synthesis by yeast promitochondria in vivo. Biochem Biophys Res Commun. 1969 Dec 4;37(6):996–1001. doi: 10.1016/0006-291x(69)90230-7. [DOI] [PubMed] [Google Scholar]
  12. Sherman F., Stewart J. W., Margoliash E., Parker J., Campbell W. The structural gene for yeast cytochrome C. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1498–1504. doi: 10.1073/pnas.55.6.1498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sherman F., Stewart J. W., Parker J. H., Inhaber E., Shipman N. A., Putterman G. J., Gardisky R. L., Margoliash E. The mutational alteration of the primary structure of yeast iso-1-cytochrome c. J Biol Chem. 1968 Oct 25;243(20):5446–5456. [PubMed] [Google Scholar]
  14. Subík J., Kovác L., Kolarov J. Oxidative phosphorylation in yeast. X. Phosphorylation ability of mutants deficient in cytochromes a and b. Biochim Biophys Acta. 1972;283(1):146–154. doi: 10.1016/0005-2728(72)90106-5. [DOI] [PubMed] [Google Scholar]
  15. Subík J., Kuzela S., Kolarov J., Kovác L., Lachowicz T. M. Oxidative phosphorylation in yeast. VI. ATPase activity and protein synthesis in mitochondria isolated from nuclear mutants deficient in cytochromes. Biochim Biophys Acta. 1970 Jun 30;205(3):513–519. doi: 10.1016/0005-2728(70)90117-9. [DOI] [PubMed] [Google Scholar]
  16. Tzagoloff A., Meagher P. Assesmbly of the mitochondrial membrane system. VI. Mitochondrial synthesis of subunit proteins of the rutamycin-sensitive adenosine triphosphatase. J Biol Chem. 1972 Jan 25;247(2):594–603. [PubMed] [Google Scholar]
  17. Weiss H., Sebald W., Bücher T. Cycloheximide resistant incorporation of amino acids into a polypeptide of the cytochrome oxidase of Neurospora crassa. Eur J Biochem. 1971 Sep 13;22(1):19–26. doi: 10.1111/j.1432-1033.1971.tb01509.x. [DOI] [PubMed] [Google Scholar]
  18. Weiss H., Ziganke B. Cytochrome b in Neurospora crassa mitochondria. Site of translation of the heme protein. Eur J Biochem. 1974 Jan 3;41(1):63–71. doi: 10.1111/j.1432-1033.1974.tb03244.x. [DOI] [PubMed] [Google Scholar]

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