Abstract
A membrane componenet of the dag transport system which serves for glycine, D-alanine, and D-serine is coded for by the dagA gene at minute 83 of the Escherichia coli chromosome. Merodiploid strains (dagA+/dagA+) show two to three times the transport activity for only those amino acids that are substrates of the dag transport system. The increased transport activity is a result of a two-to threefold increase in Vmax for amino acid uptake with little or no change in the Km value. The two- to threefold gene dose effect of the merodiploid strains is maintained even during carbon starvation, eliminating the possibility that a greater energy supply for transport activity may account for the effect. Since merodiploids which carry more than one copy of the dagA allele show a gene dose response for transport activity, we conclude that the membrane componenet of the dag transport system which is coded for by the dagA allele is present in limiting amounts.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cosloy S. D. D-serine transport system in Escherichia coli K-12. J Bacteriol. 1973 May;114(2):679–684. doi: 10.1128/jb.114.2.679-684.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Curtiss R., 3rd, Charamella L. J., Berg C. M., Harris P. E. Kinetic and genetic analyses of D-cycloserine inhibition and resistance in Escherichia coli. J Bacteriol. 1965 Nov;90(5):1238–1250. doi: 10.1128/jb.90.5.1238-1250.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox C. F. A lipid requirement for induction of lactose transport in Escherichia coli. Proc Natl Acad Sci U S A. 1969 Jul;63(3):850–855. doi: 10.1073/pnas.63.3.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KESSEL D., LUBIN M. STABILITY OF ALPHA-HYDROGEN OF AMINO ACIDS DURING ACTIVE TRANSPORT. Biochemistry. 1965 Mar;4:561–565. doi: 10.1021/bi00879a029. [DOI] [PubMed] [Google Scholar]
- Kaback H. R., Kostellow A. B. Glycine uptake in Escherichia coli. I. Glycine uptake by whole cells of Escherichia coli W+ and a D-serine-resistant. J Biol Chem. 1968 Apr 10;243(7):1384–1389. [PubMed] [Google Scholar]
- Kennedy E. P., Rumley M. K., Armstrong J. B. Dierect measurement of the binding of labeled sugars to the lactose permease M protein. J Biol Chem. 1974 Jan 10;249(1):33–37. [PubMed] [Google Scholar]
- Lombardi F. J., Kaback H. R. Mechanisms of active transport in isolated bacterial membrane vesicles. 8. The transport of amino acids by membranes prepared from Escherichia coli. J Biol Chem. 1972 Dec 25;247(24):7844–7857. [PubMed] [Google Scholar]
- Oxender D. L. Membrane transport. Annu Rev Biochem. 1972;41(10):777–814. doi: 10.1146/annurev.bi.41.070172.004021. [DOI] [PubMed] [Google Scholar]
- Piperno J. R., Oxender D. L. Amino acid transport systems in Escherichia coli K-12. J Biol Chem. 1968 Nov 25;243(22):5914–5920. [PubMed] [Google Scholar]
- Robbins J. C., Oxender D. L. Transport systems for alanine, serine, and glycine in Escherichia coli K-12. J Bacteriol. 1973 Oct;116(1):12–18. doi: 10.1128/jb.116.1.12-18.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stubbs J., Horwitz A., Moses V. Studies on beta-galactoside transport in a Proteus mirabilis merodiploid carrying an Escherichia coli lactose operon. J Bacteriol. 1973 Oct;116(1):131–140. doi: 10.1128/jb.116.1.131-140.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wargel R. J., Hadur C. A., Neuhaus F. C. Mechanism of D-cycloserine action: transport mutants for D-alanine, D-cycloserine, and glycine. J Bacteriol. 1971 Mar;105(3):1028–1035. doi: 10.1128/jb.105.3.1028-1035.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wargel R. J., Shadur C. A., Neuhaus F. C. Mechanism of D-cycloserine action: transport systems for D-alanine, D-cycloserine, L-alanine, and glycine. J Bacteriol. 1970 Sep;103(3):778–788. doi: 10.1128/jb.103.3.778-788.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]