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. 1973 Jul;115(1):73–75. doi: 10.1128/jb.115.1.73-75.1973

Fatty Acid Composition of L-Forms of Streptococcus faecalis Cultured at Different Osmolalities

J Z Montgomerie 1,2,3, G M Kalmanson 1,2,3, L B Guze 1,2,3
PMCID: PMC246214  PMID: 4197910

Abstract

The fatty acid composition of the membranes of three different penicillin-produced L-forms of Streptococcus faecalis was determined: (i) a stable (nonreverting) L-form (T53) cultured in brain heart infusion (BHI) with 0.5 M sucrose; (ii) a stable L-form (T531) cultured in BHI without sucrose; and (iii) an unstable L-form (T9) cultured in BHI with 0.5 M sucrose and 1,000 U of penicillin per ml. L-forms were obtained by centrifugation and lysed by washing in 1 mM tris(hydroxymethyl)aminomethane-hydrochloride buffer. The parent S. faecalis was also cultured in BHI and BHI containing 0.5 M sucrose, and washed with buffer. The fatty acid composition of L-forms of S. faecalis cultured in BHI without sucrose (370 mosmol) had higher C18:1 and lower C18 than L-forms cultured in the same media with added 0.5 M sucrose (950 mosmol) in both exponential and stationary cultures. In the stationary phase of growth, C19 was reduced in the L-forms cultured without sucrose. Similar changes were seen in the parent S. faecalis cultured in the two types of media. These changes in membrane fatty acids may relate to osmo-regulation of the L-forms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clasener H. A., Ensering H. L., Hijmans W. Persistance in mice of the L-phase of three streptococcal strains adapted to physiological osmotic conditions. J Gen Microbiol. 1970 Aug;62(2):195–202. doi: 10.1099/00221287-62-2-195. [DOI] [PubMed] [Google Scholar]
  2. Montgomerie J. Z., Kalmanson G. M., Guze L. B. The effects of antibiotics on the protoplast and bacterial forms of Streptococcus faecalis. J Lab Clin Med. 1966 Oct;68(4):543–551. [PubMed] [Google Scholar]
  3. Montgomerie J. Z., Kalmanson G. M., Hubert E. G., Guze L. B. Osmotic stability and sodium and potassium content of L-forms of Streptococcus faecalis. J Bacteriol. 1972 May;110(2):624–627. doi: 10.1128/jb.110.2.624-627.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. O'Brien J. S., Fillerup D. L., Mead J. F. Quantification and fatty acid and fatty aldehyde composition of ethanolamine, choline, and serine glycerophosphatides in human cerebral grey and white matter. J Lipid Res. 1964 Jul;5(3):329–338. [PubMed] [Google Scholar]
  5. Panos C., Cohen M., Fagan G. Lipid alterations after cell wall inhibition. Fatty acid content of Streptococcus pyogenes and derived L-form. Biochemistry. 1966 May;5(5):1461–1468. doi: 10.1021/bi00869a003. [DOI] [PubMed] [Google Scholar]
  6. Razin S., Tourtellotte M. E., McElhaney R. N., Pollack J. D. Influence of lipid components of Mycoplasma laidlawii membranes on osmotic fragility of cells. J Bacteriol. 1966 Feb;91(2):609–616. doi: 10.1128/jb.91.2.609-616.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. VORBECK M. L., MARINETTI G. V. SEPARATION OF GLYCOSYL DIGLYCERIDES FROM PHOSPHATIDES USING SILICIC ACID COLUMN CHROMATOGRAPHY. J Lipid Res. 1965 Jan;6:3–6. [PubMed] [Google Scholar]
  8. WALKER B. L., KUMMEROW F. A. ERYTHROCYTE FATTY ACID COMPOSITION AND APPARENT PERMEABILITY TO NON-ELECTROLYTES. Proc Soc Exp Biol Med. 1964 Apr;115:1099–1103. doi: 10.3181/00379727-115-29126. [DOI] [PubMed] [Google Scholar]
  9. de Gier J., Mandersloot J. G., van Deenen L. L. Lipid composition and permeability of liposomes. Biochim Biophys Acta. 1968 Jun 11;150(4):666–675. doi: 10.1016/0005-2736(68)90056-4. [DOI] [PubMed] [Google Scholar]

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