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. 1973 Jul;115(1):82–87. doi: 10.1128/jb.115.1.82-87.1973

Localization of a Binding Site for Ribosomal Protein S8 Within the 16S Ribosomal Ribonucleic Acid of Escherichia coli

H W Schaup a, M L Sogin a, C G Kurland a,1, C R Woese a
PMCID: PMC246216  PMID: 4577755

Abstract

A region of 16S ribosomal ribonucleic acid that binds Escherichia coli ribosomal protein S8 has been isolated and characterized. It corresponds to what has been designated as fragment C of the ribonucleic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  2. Cory S., Adams J. M., Spahr P. F., Rensing U. Sequence of 51 nucleotides at the 3'-end of R17 bacteriophage RNA. J Mol Biol. 1972 Jan 14;63(1):41–56. doi: 10.1016/0022-2836(72)90520-7. [DOI] [PubMed] [Google Scholar]
  3. Fellner P., Ehresmann C., Ebel J. P. Nucleotide sequences present within the 16S ribosomal RNA of Escherichia coli. Nature. 1970 Jan 3;225(5227):26–29. doi: 10.1038/225026a0. [DOI] [PubMed] [Google Scholar]
  4. Fellner P., Ehresmann C., Stiegler P., Ebel J. P. Partial nucleotide sequence of 16S ribosomal RNA from E. coli. Nat New Biol. 1972 Sep 6;239(88):1–5. [PubMed] [Google Scholar]
  5. Hardy S. J., Kurland C. G., Voynow P., Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969 Jul;8(7):2897–2905. doi: 10.1021/bi00835a031. [DOI] [PubMed] [Google Scholar]
  6. Hecht N. B., Woese C. R. Separation of bacterial ribosomal ribonucleic acid from its macromolecular precursors by polyacrylamide gel electrophoresis. J Bacteriol. 1968 Mar;95(3):986–990. doi: 10.1128/jb.95.3.986-990.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Nomura M., Mizushima S., Ozaki M., Traub P., Lowry C. V. Structure and function of ribosomes and their molecular components. Cold Spring Harb Symp Quant Biol. 1969;34:49–61. doi: 10.1101/sqb.1969.034.01.009. [DOI] [PubMed] [Google Scholar]
  9. Schaup H. W., Green M., Kurland C. G. Molecular interactions of ribosomal components. II. Site-specific complex formation between 30S proteins and ribosomal RNA. Mol Gen Genet. 1971;112(1):1–8. doi: 10.1007/BF00266926. [DOI] [PubMed] [Google Scholar]
  10. Schaup H. W., Kurland C. G. Molecular interactions of ribosomal components. 3. Isolation of the RNA binding site for a ribosomal protein. Mol Gen Genet. 1972;114(4):350–357. doi: 10.1007/BF00267503. [DOI] [PubMed] [Google Scholar]
  11. Schaup H. W., Sogin M., Woese C. Characterization of an RNA "binding site" for a specific ribosomal protein of Escherichia coli. Mol Gen Genet. 1972;114(1):1–8. doi: 10.1007/BF00268740. [DOI] [PubMed] [Google Scholar]
  12. Sogin M., Pace B., Pace N. R., Woese C. R. Primary structural relationship of p16 to m16 ribosomal RNA. Nat New Biol. 1971 Jul 14;232(28):48–49. doi: 10.1038/newbio232048a0. [DOI] [PubMed] [Google Scholar]
  13. Traub P., Nomura M. Structure and function of E. coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and proteins. Proc Natl Acad Sci U S A. 1968 Mar;59(3):777–784. doi: 10.1073/pnas.59.3.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zimmermann R. A., Muto A., Fellner P., Ehresmann C., Branlant C. Location of ribosomal protein binding sites on 16S ribosomal RNA. Proc Natl Acad Sci U S A. 1972 May;69(5):1282–1286. doi: 10.1073/pnas.69.5.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]

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