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. 1973 Jul;115(1):121–128. doi: 10.1128/jb.115.1.121-128.1973

A Regulatory Gene of Phenylalanine Biosynthesis (pheR) in Salmonella typhimurium

E G Gollub 1, K P Liu 1, D B Sprinson 1
PMCID: PMC246221  PMID: 4577738

Abstract

4-Fluorophenylalanine-resistant mutants of Salmonella typhimurium were isolated in which synthesis of chorismate mutase P-prephenate dehydratase (specified by pheA) was highly elevated. Transduction analysis showed that the mutation affecting pheA activity was not linked to pheA, and conjugation and merodiploid analysis indicated that it was in the 95- to 100-min region of the Salmonella chromosome. Evidence is presented for the hypothesis that the mutation responsible for constitutivity of chorismate mutase P-prephenate dehydratase occurred in pheR, a gene specifying a cytoplasmic product that affected pheA. pheR mutants were found to carry a second mutation, tyrO. The tyrO mutation acts cis to cause increased levels of the tyrosine biosynthetic enzymes 3-deoxy-d-arabinoheptulosonate 7-phosphate synthetase (tyr) and prephenate dehydrogenase, but it has no effect on regulation of pheA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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