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. 1973 Jul;115(1):205–212. doi: 10.1128/jb.115.1.205-212.1973

A Bifunctional Enzyme in Pseudomonas aeruginosa: a New Pattern in the Organization of Enzymes Concerned with Phenylalanine and Tyrosine Biosynthesis

S I Ahmed a,1, J J R Campbell a
PMCID: PMC246231  PMID: 4197900

Abstract

Two isozymes of chorismate mutase (CA mutase1 and CA mutase2) and two isozymes of prephenate dehydratase (PPA dehydratase1 and PPA dehydratase2) have been found in Pseudomonas aeruginosa. The activities CA mutase2-PPA dehydratase2 catalyzing phenylalanine biosynthesis have been purified almost 40-fold and were found to be associated as a bifunctional enzyme or an enzyme complex. The enzymes specific for tyrosine biosynthesis did not appear to manifest such physical association. Thus, the organization of enzymes concerned with phenylalanine and tyrosine biosynthesis in P. aeruginosa is unique and is unlike most other organisms. Single site mutants have been isolated which have lost both CA mutase2-PPA dehydratase2 activities resulting in a requirement for phenylalanine for growth. Single site revertants of these mutants regained both these activities simultaneously and were able to grow on minimal medium. A mutant, r6, was also isolated which had normal CA mutase2 but lacked PPA dehydratase2 activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDREWS P. Estimation of molecular weights of proteins by gel filtration. Nature. 1962 Oct 6;196:36–39. doi: 10.1038/196036a0. [DOI] [PubMed] [Google Scholar]
  2. CERUTTI P., GUROFF G. ENZYMATIC FORMATION OF PHENYLPYRUVIC ACID IN PSEUDOMONAS SP. (ATCC 11299A) AND ITS REGULATION. J Biol Chem. 1965 Jul;240:3034–3038. [PubMed] [Google Scholar]
  3. COTTON R. G., GIBSON F. THE BIOSYNTHESIS OF PHENYLALANINE AND TYROSINE; ENZYMES CONVERTING CHORISMIC ACID INTO PREPHENIC ACID AND THEIR RELATIONSHIPS TO PREPHENATE DEHYDRATASE AND PREPHENATE DEHYDROGENASE. Biochim Biophys Acta. 1965 Apr 12;100:76–88. doi: 10.1016/0304-4165(65)90429-0. [DOI] [PubMed] [Google Scholar]
  4. Cotton R. G., Gibson F. The biosynthesis of phenylalanine and tyrosine in the pea (Pisum sativum): chorismate mutase. Biochim Biophys Acta. 1968 Feb 1;156(1):187–189. doi: 10.1016/0304-4165(68)90118-9. [DOI] [PubMed] [Google Scholar]
  5. Dayan J., Sprinson D. B. Enzyme alterations in tyrosine and phenylalanine auxotrophs of Salmonella typhimurium. J Bacteriol. 1971 Dec;108(3):1174–1180. doi: 10.1128/jb.108.3.1174-1180.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giles N. H., Partridge C. W., Ahmed S. I., Case M. E. The occurrence of two dehydroquinases in Neurospora crassa, one constitutive and one inducible. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1930–1937. doi: 10.1073/pnas.58.5.1930. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Koch G. L., Shaw D. C., Gibson F. Characterisation of the subunits of chorismate mutase-prephenate dehydrogenase from Escherichia coli K12. Biochim Biophys Acta. 1971 Mar 23;229(3):805–812. doi: 10.1016/0005-2795(71)90299-6. [DOI] [PubMed] [Google Scholar]
  8. Koch G. L., Shaw D. C., Gibson F. Studies on the subunit structure of chorismate mutase-prephenate dehydrogenase from Aerobacter aerogenes. Biochim Biophys Acta. 1970 Sep 16;212(3):387–395. doi: 10.1016/0005-2744(70)90244-5. [DOI] [PubMed] [Google Scholar]
  9. Koch G. L., Shaw D. C., Gibson F. The purification and characterisation of chorismate mutase-prephenate dehydrogenase from Escherichia coli K12. Biochim Biophys Acta. 1971 Mar 23;229(3):795–804. doi: 10.1016/0005-2795(71)90298-4. [DOI] [PubMed] [Google Scholar]
  10. Koch G. L., Shaw D. C., Gibson F. Tyrosine biosynthesis in Aerobacter aerogenes. Purification and properties of chorismate mutase-prephenate dehydrogenase. Biochim Biophys Acta. 1970 Sep 16;212(3):375–386. doi: 10.1016/0005-2744(70)90243-3. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Longley R. P., Halliwell J. E., Campbell J. J., Ingledew W. M. The branchpoint of pyocyanine biosynthesis. Can J Microbiol. 1972 Sep;18(9):1357–1363. doi: 10.1139/m72-210. [DOI] [PubMed] [Google Scholar]
  13. Lorence J. H., Nester E. W. Multiple molecular forms of chorismate mutase in Bacillus subtillis. Biochemistry. 1967 May;6(5):1541–1553. doi: 10.1021/bi00857a041. [DOI] [PubMed] [Google Scholar]
  14. Nester E. W., Jensen R. A. Control of aromatic acid biosynthesis in Bacillus subtilis: sequenial feedback inhibition. J Bacteriol. 1966 Apr;91(4):1594–1598. doi: 10.1128/jb.91.4.1594-1598.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  16. el-Eryani A. A. Genetic control of phenylalanine and tyrosine biosynthesis in Neurospora crassa. Genetics. 1969 Jul;62(3):711–723. [PMC free article] [PubMed] [Google Scholar]

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