Abstract
The extreme sensitivity of polA− cells to thymineless death is due, primarily, to the absence of an extended lag prior to the commencement of death. Once thymineless death has commenced, the rate in polA− cells is only slightly faster than in polA+ cells.
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Selected References
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- BARNER H. D., COHEN S. S. The induction of thymine synthesis by T2 infection of a thymine requiring mutant of Escherichia coli. J Bacteriol. 1954 Jul;68(1):80–88. doi: 10.1128/jb.68.1.80-88.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berg C. M. Auxotroph accumulation in deoxyribonucleic acid polymeraseless strains of Escherichia coli K-12. J Bacteriol. 1971 Jun;106(3):797–801. doi: 10.1128/jb.106.3.797-801.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Breitman T. R., Maury P. B., Toal J. N. Loss of deoxyribonucleic acid-thymine during thymine starvation of Escherichia coli. J Bacteriol. 1972 Oct;112(1):646–648. doi: 10.1128/jb.112.1.646-648.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cummings D. J., Kusy A. R. Thymineless death in Escherichia coli: deoxyribonucleic acid replication and the immune state. J Bacteriol. 1970 Apr;102(1):106–117. doi: 10.1128/jb.102.1.106-117.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
- MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
- Sedgwick S. G., Bridges B. A. Alkaline sucrose gradient sedimentation of chromosomal deoxyribonucleic acid from Escherichia coli PolA + and PolA - strains during thymine starvation. J Bacteriol. 1971 Dec;108(3):1422–1423. doi: 10.1128/jb.108.3.1422-1423.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smirnov G. B., Favorskaya Y. N., Skavronskaya A. G. Monofunctional alkylating agent-induced inactivation, mutagenesis and DNA degradation in an Escherichia coli mutant deficient in DNA polymerase. Mol Gen Genet. 1971;111(4):357–367. doi: 10.1007/BF00569788. [DOI] [PubMed] [Google Scholar]