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. 1973 Sep;115(3):848–857. doi: 10.1128/jb.115.3.848-857.1973

Deoxyribonucleic Acid-Dependent Ribonucleic Acid Polymerase of Caulobacter crescentus

Ina K Bendis 1, Lucille Shapiro 1
PMCID: PMC246328  PMID: 4734061

Abstract

Deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase (EC 2.7.7.6) was purified from the dimorphic bacterium Caulobacter crescentus at three stages in development. Enzyme from pure populations of stalked cells, as well as populations enriched in swarmer and predivisional cells, appeared identical in subunit structure and template requirements. The molecular weights of the enzyme subunits were 165,000, 155,000, 101,000, and 44,000, respectively. By analogy with RNA polymerase from other bacterial sources, they are considered to be components of the C. crescentus holoenzyme, β′, β, σ, and α, respectively. The C. crescentus enzyme appeared similar to the Pseudomonas aeruginosa enzyme and unlike the Escherichia coli enzyme with respect to subunit molecular weights and failure to separate into core and sigma components upon phosphocellulose chromatography. In addition, the effects of ionic strength on the time course of polymerization varied both with the sources of bacterial polymerase and bacteriophage DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Agabian-Keshishian N., Shapiro L. Stalked bacteria: properties of deoxriybonucleic acid bacteriophage phiCbK. J Virol. 1970 Jun;5(6):795–800. doi: 10.1128/jvi.5.6.795-800.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Burgess R. R., Travers A. A., Dunn J. J., Bautz E. K. Factor stimulating transcription by RNA polymerase. Nature. 1969 Jan 4;221(5175):43–46. doi: 10.1038/221043a0. [DOI] [PubMed] [Google Scholar]
  3. Degnen S. T., Newton A. Chromosome replication during development in Caulobacter crescentus. J Mol Biol. 1972 Mar 14;64(3):671–680. doi: 10.1016/0022-2836(72)90090-3. [DOI] [PubMed] [Google Scholar]
  4. Greenleaf A. L., Linn T. G., Losick R. Isolation of a new RNA polymerase-binding protein from sporulating Bacillus subtilis. Proc Natl Acad Sci U S A. 1973 Feb;70(2):490–494. doi: 10.1073/pnas.70.2.490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnson J. C., DeBacker M., Boezi J. A. Deoxyribonucleic acid-dependent ribonucleic acid polymerase of Pseudomonas putida. J Biol Chem. 1971 Mar 10;246(5):1222–1232. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Losick R., Shorenstein R. G., Sonenshein A. L. Structural alteration of RNA polymerase during sporulation. Nature. 1970 Aug 29;227(5261):910–913. doi: 10.1038/227910a0. [DOI] [PubMed] [Google Scholar]
  8. Losick R., Sonenshein A. L. Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis. Nature. 1969 Oct 4;224(5214):35–37. doi: 10.1038/224035a0. [DOI] [PubMed] [Google Scholar]
  9. Newton A. Role of transcription in the temporal control of development in Caulobacter crescentus (stalk-rifampin-RNA synthesis-DNA synthesis-motility). Proc Natl Acad Sci U S A. 1972 Feb;69(2):447–451. doi: 10.1073/pnas.69.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. POINDEXTER J. S. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP. Bacteriol Rev. 1964 Sep;28:231–295. doi: 10.1128/br.28.3.231-295.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schmidt J. M., Stanier R. Y. The development of cellular stalks in bacteria. J Cell Biol. 1966 Mar;28(3):423–436. doi: 10.1083/jcb.28.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  13. Shapiro L., Agabian-Keshishian N., Bendis I. Bacterial differentiation. Science. 1971 Sep 3;173(4000):884–892. doi: 10.1126/science.173.4000.884. [DOI] [PubMed] [Google Scholar]
  14. Shapiro L., Agabian-Keshishian N. Specific Assay for Differentiation in the Stalked Bacterium Caulobacter crescentus. Proc Natl Acad Sci U S A. 1970 Sep;67(1):200–203. doi: 10.1073/pnas.67.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shapiro L., Maizel J. V., Jr Synthesis and structure of Caulobacter crescentus flagella. J Bacteriol. 1973 Jan;113(1):478–485. doi: 10.1128/jb.113.1.478-485.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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