Abstract
Efficiency of adenosine triphosphate (ATP) formation from glucose oxidation in Aphanocapsa 6714 was estimated by quantitative measurement of phosphorylated intermediary metabolites and glycogen (polyglucose) formed from 14C-glucose. P/2e ratios based on oxygen uptake ranged from 2.62 to 3.08, whereas those based on 14CO2 evolution ranged from 1.66 to 1.72. The synthesis of glycogen, which is the dominant energy-consuming process in resting cells exposed to exogenous glucose, was almost totally inhibited under anaerobic conditions, and the cellular concentration of ATP decreased steadily. Thus, both net synthesis of ATP and the steady-state concentration of ATP are obligatorily linked to respiration in this heterotrophic unicellular blue-green alga.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BASSHAM J. A., KIRK M. PHOTOSYNTHESIS OF AMINO ACIDS. Biochim Biophys Acta. 1964 Sep 4;90:553–562. doi: 10.1016/0304-4165(64)90234-x. [DOI] [PubMed] [Google Scholar]
- Hempfling W. P., Vishniac W. Oxidative phosphorylation in extracts of thiobacillus X. Biochem Z. 1965 Aug 6;342(3):272–287. [PubMed] [Google Scholar]
- MOSES V., LONBERG-HOLM K. K. A semiautomatic device for measuring radioactivity on two-dimensional paper chromatograms. Anal Biochem. 1963 Jan;5:11–27. doi: 10.1016/0003-2697(63)90053-8. [DOI] [PubMed] [Google Scholar]
- Pelroy R. A., Bassham J. A. Photosynthetic and dark carbon metabolism in unicellular blue-green algae. Arch Mikrobiol. 1972;86(1):25–38. doi: 10.1007/BF00412397. [DOI] [PubMed] [Google Scholar]
- Pelroy R. A., Rippka R., Stanier R. Y. Metabolism of glucose by unicellular blue-green algae. Arch Mikrobiol. 1972;87(4):303–322. doi: 10.1007/BF00409131. [DOI] [PubMed] [Google Scholar]
- Smith A. J., London J., Stanier R. Y. Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli. J Bacteriol. 1967 Oct;94(4):972–983. doi: 10.1128/jb.94.4.972-983.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanier R. Y., Kunisawa R., Mandel M., Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev. 1971 Jun;35(2):171–205. doi: 10.1128/br.35.2.171-205.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]