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. 1973 Sep;115(3):1094–1102. doi: 10.1128/jb.115.3.1094-1102.1973

tyrR, A Regulatory Gene of Tyrosine Biosynthesis in Salmonella typhimurium

E G Gollub 1, K P Liu 1, D B Sprinson 1
PMCID: PMC246358  PMID: 4147003

Abstract

4-Fluorophenylalanine-resistant mutants of Salmonella typhimurium were isolated in which tyrosine pathway enzymes were not repressed by l-tyrosine. The mutants produced elevated levels of 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and chorismate mutase T-prephenate dehydrogenase, and these enzymes as well as transaminase A were not repressed by high concentrations of tyrosine. Genetic analysis revealed that a mutation in a gene designated tyrR was responsible for the constitutivity of the tyrosine pathway enzymes in strains SG1, SG7, and SG9, and that tyrR was linked to pyrF. In strain SG1 a mutation had also occurred in aroF, the structural gene for DAHP synthetase (tyr), resulting in loss of sensitivity of this enzyme to end-product inhibition. There appeared to be no relationship between loss of feedback inhibition and loss of end-product repression, since derivative strains of SG1 that carried only the tyrR mutation behaved like the singly mutated tyrR strains, SG7 and SG9, in showing high constitutive levels of tyrosine-specific enzymes that were not repressed by tyrosine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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