Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Oct;116(1):54–58. doi: 10.1128/jb.116.1.54-58.1973

Isolation and Characterization of a Mutant of Salmonella typhimurium Deficient in a Major Deoxyribonucleic Acid Polymerase Activity

Harvey J Whitfield a, Gary Levine a,1
PMCID: PMC246390  PMID: 4355486

Abstract

A mutant of Salmonella typhimurium strain LT2 that is deficient in a major deoxyribonucleic acid (DNA) polymerase activity has been isolated and characterized. This mutant resembles the pol mutants of E. coli in that it has low DNA polymerase activity and it is sensitive to methyl methane sulfonate as well as ultraviolet irradiation. Revertants selected for methyl methane sulfonate resistance are no longer sensitive to ultraviolet irradiation and contain normal DNA polymerase levels. No direct role in replication can be ascribed to this polymerase activity since cells grow well in its absence. In addition, the LT2 plasmid has been shown to exist in the mutant strain.

Full text

PDF
54

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Botstein D., Matz M. J. A recombination function essential to the growth of bacteriophage P22. J Mol Biol. 1970 Dec 28;54(3):417–440. doi: 10.1016/0022-2836(70)90119-1. [DOI] [PubMed] [Google Scholar]
  2. Campbell J. L., Soll L., Richardson C. C. Isolation and partial characterization of a mutant of Escherichia coli deficient in DNA polymerase II. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2090–2094. doi: 10.1073/pnas.69.8.2090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  5. Dowman J. E., Meynell G. G. Pleiotropic effects of de-repressed bacterial sex factors on colicinogeny and cell wall structure. Mol Gen Genet. 1970;109(1):57–68. doi: 10.1007/BF00334046. [DOI] [PubMed] [Google Scholar]
  6. Gross J. D. DNA replication in bacteria. Curr Top Microbiol Immunol. 1972;57:39–74. doi: 10.1007/978-3-642-65297-4_2. [DOI] [PubMed] [Google Scholar]
  7. Gross J., Gross M. Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1166–1168. doi: 10.1038/2241166a0. [DOI] [PubMed] [Google Scholar]
  8. Kelley W. S., Whitfield H. J. Purification of an altered DNA polymerase from an E. coli strain with a pol mutation. Nature. 1971 Mar 5;230(5288):33–36. doi: 10.1038/230033a0. [DOI] [PubMed] [Google Scholar]
  9. Kornberg A. Active center of DNA polymerase. Science. 1969 Mar 28;163(3874):1410–1418. doi: 10.1126/science.163.3874.1410. [DOI] [PubMed] [Google Scholar]
  10. Kornberg T., Gefter M. L. Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II. Proc Natl Acad Sci U S A. 1971 Apr;68(4):761–764. doi: 10.1073/pnas.68.4.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Laipis P. J., Ganesan A. T. A deoxyribonucleic acid polymerase I-deficient mutant of Bacillus subtilis. J Biol Chem. 1972 Sep 25;247(18):5867–5871. [PubMed] [Google Scholar]
  13. Monk M., Kinross J. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J Bacteriol. 1972 Mar;109(3):971–978. doi: 10.1128/jb.109.3.971-978.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sanderson K. E. Linkage map of Salmonella typhimurium, edition IV. Bacteriol Rev. 1972 Dec;36(4):558–586. doi: 10.1128/br.36.4.558-586.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith H. O., Levine M. A phage P22 gene controlling integration of prophage. Virology. 1967 Feb;31(2):207–216. doi: 10.1016/0042-6822(67)90164-x. [DOI] [PubMed] [Google Scholar]
  17. Spratt B. G. Replication of extrachromosomal elements in a DNA synthesis initiation mutant of Salmonella typhimurium. Biochem Biophys Res Commun. 1972 Aug 7;48(3):496–501. doi: 10.1016/0006-291x(72)90375-0. [DOI] [PubMed] [Google Scholar]
  18. Tafler S. W., Setlow P., Levine L. Serological relatedness of bacterial deoxyribonucleic acid polymerases. J Bacteriol. 1973 Jan;113(1):18–23. doi: 10.1128/jb.113.1.18-23.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woodworth-Gutai M., Israel V., Levine M. New deoxyribonuclease activity after bacteriophage P22 infection. J Virol. 1972 May;9(5):746–751. doi: 10.1128/jvi.9.5.746-751.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yamagami H., Yamamoto N. Contribution of the bacterial recombination function to replication of bacteriophage P2. J Mol Biol. 1970 Oct 28;53(2):281–285. doi: 10.1016/0022-2836(70)90300-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES