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. 1973 Oct;116(1):146–153. doi: 10.1128/jb.116.1.146-153.1973

Conditions Affecting the Isolation from Transformed Cells of Bacillus subtilis of High-Molecular-Weight Single-Stranded Deoxyribonucleic Acid of Donor Origin

R Davidoff-Abelson 1, D Dubnau 1
PMCID: PMC246402  PMID: 4200839

Abstract

The deoxyribonucleic acid (DNA) extraction procedure of Piechowska and Fox was evaluated to determine which steps are required for the isolation of high-molecular-weight single-stranded material from transformed cultures of Bacillus subtilis. The results indicate that high-molecular-weight single-stranded DNA can be isolated when certain basic proteins are present at the time of lysis. In the absence of such protective agents as lysozyme or cytC the single-stranded DNA is degraded. The single-stranded DNA can also be protected by being treated with lysozyme at low temperature. The high molecular weight of this single-stranded material and its kinetics of appearance are consistent with its being an intermediate in the transformation process.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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