Abstract
In growing cultures of Escherichia coli, the nonacylated glycerol of phosphatidylglycerol (PG) is labeled more rapidly than is the acylated glycerol. This is, in part, due to a rapid exchange reaction of the nonacylated glycerol. Only some of the PG molecules undergo this reaction while others are stable. Using a mutant unable to make glycerophosphate, we have shown that the nonacylated glycerol of PG can exchange with non-phosphorylated glycerol.
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Selected References
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- Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- Ballesta J. P., Schaechter M. Effect of shift-down and growth inhibition on phospholipid metabolism of Escherichia coli. J Bacteriol. 1971 Jul;107(1):251–258. doi: 10.1128/jb.107.1.251-258.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang Y. Y., Kennedy E. P. Biosynthesis of phosphatidyl glycerophosphate in Escherichia coli. J Lipid Res. 1967 Sep;8(5):447–455. [PubMed] [Google Scholar]
- Chang Y. Y., Kennedy E. P. Pathways for the synthesis of glycerophosphatides in Escherichia coli. J Biol Chem. 1967 Feb 10;242(3):516–519. [PubMed] [Google Scholar]
- Daniels M. J. Some features of the bacterial membrane studied with the aid of a new fractionation technique. Biochem J. 1971 Apr;122(2):197–207. doi: 10.1042/bj1220197. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAYASHI S., KOCH J. P., LIN E. C. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed] [Google Scholar]
- KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B. J Biol Chem. 1963 Sep;238:2919–2922. [PubMed] [Google Scholar]
- Kanemasa Y., Akamatsu Y., Nojima S. Composition and turnover of the phospholipids in Escherichia coli. Biochim Biophys Acta. 1967 Oct 2;144(2):382–390. [PubMed] [Google Scholar]
- Kundig W., Roseman S. Sugar transport. II. Characterization of constitutive membrane-bound enzymes II of the Escherichia coli phosphotransferase system. J Biol Chem. 1971 Mar 10;246(5):1407–1418. [PubMed] [Google Scholar]
- Lusk J. E., Kennedy E. P. Altered phospholipid metabolism in a sodium-sensitive mutant of Escherichia coli. J Bacteriol. 1972 Mar;109(3):1034–1046. doi: 10.1128/jb.109.3.1034-1046.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McQUILLEN K., ROBERTS R. B. The utilization of acetate for synthesis in Escherichia coli. J Biol Chem. 1954 Mar;207(1):81–95. [PubMed] [Google Scholar]
- Milner L. S., Kaback H. R. The role of phosphatidylglycerol in the vectorial phosphorylation of sugar by isolated bacterial membrane preparations. Proc Natl Acad Sci U S A. 1970 Mar;65(3):683–690. doi: 10.1073/pnas.65.3.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ono Y., White D. C. Consequences of the inhibition of cardiolipin metabolism in Haemophilus parainfluenzae. J Bacteriol. 1971 Dec;108(3):1065–1071. doi: 10.1128/jb.108.3.1065-1071.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothfield L., Finkelstein A. Membrane biochemistry. Annu Rev Biochem. 1968;37:463–496. doi: 10.1146/annurev.bi.37.070168.002335. [DOI] [PubMed] [Google Scholar]
- Rothfield L., Pearlman-Kothencz M. Synthesis and assembly of bacterial membrane components. A lipopolysaccharide-phospholipid-protein complex excreted by living bacteria. J Mol Biol. 1969 Sep 28;44(3):477–492. doi: 10.1016/0022-2836(69)90374-x. [DOI] [PubMed] [Google Scholar]
- TARLOV A. R., KENNEDY E. P. THE BETA-GALACTOSIDE PERMEASE SYSTEM AND THE METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI. J Biol Chem. 1965 Jan;240:49–53. [PubMed] [Google Scholar]
- Tucker A. N., White D. C. Detection of a rapidly metabolizing portion of the membrane cardiolipin in Haemophilus parainfluenzae. J Bacteriol. 1971 Dec;108(3):1058–1064. doi: 10.1128/jb.108.3.1058-1064.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tucker A. N., White D. C. Heterogeneity of phospholipid composition in the bacterial membrane. J Bacteriol. 1970 May;102(2):508–513. doi: 10.1128/jb.102.2.508-513.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White D. C., Tucker A. N. Phospholipid metabolism during bacterial growth. J Lipid Res. 1969 Mar;10(2):220–233. [PubMed] [Google Scholar]