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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Oct;116(1):226–229. doi: 10.1128/jb.116.1.226-229.1973

Cell Growth and Division in Escherichia coli: a Common Genetic Control Involved in Cell Division and Minicell Formation

George G Khachatourians a,1, D Joseph Clark a,2, Howard I Adler a, Alice A Hardigree a
PMCID: PMC246412  PMID: 4583211

Abstract

Escherichia coli Div 124(ts) is a conditional-lethal cell division mutant formed from a cross between a mutant that produces polar anucleated minicells and a temperature-sensitive cell division mutant affected in a stage of cross-wall synthesis. Under permissive growth temperature (30 C), Div 124(ts) grows and produces normal progeny cells and anucleated minicells from its polar ends. When transferred to nonpermissive growth temperature (42 C), growth and macromolecular synthesis continue, but cell division and minicell formation are inhibited. Growth at 42 C results in formation of filamentous cells showing some constrictions along the length of the filaments. Return of the filaments from 42 to 30 C results in cell division and minicell formation in association with the constrictions and other areas along the length of the filaments. This gives rise to a “necklace-type” array of cells and minicells. Recovery of cell division is observed after a lag and is followed by a burst in cell division and finally by a return to the normal growth characteristic of 30 C cultures. Recovery of cell division takes place in the presence of chloramphenicol or nalidixic acid when these are added at the time of shift from 42 to 30 C, and indicates that a division potential for filament fragmentation is accumulated while the cells are at 42 C. This division potential is used for the production of both minicells and cells of normal length. The conditional-lethal temperature sensitive mutation controls a step(s) in cross-wall synthesis common to cell division and minicell formation.

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Selected References

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  1. ADLER H. I., HARDIGREE A. A. ANALYSIS OF A GENE CONTROLLING CELL DIVISION AND SENSITIVITY TO RADIATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Mar;87:720–726. doi: 10.1128/jb.87.3.720-726.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark D. J. Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B-r. J Bacteriol. 1968 Oct;96(4):1214–1224. doi: 10.1128/jb.96.4.1214-1224.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Higgins M. L., Shockman G. D. Procaryotic cell division with respect to wall and membranes. CRC Crit Rev Microbiol. 1971 May;1(1):29–72. doi: 10.3109/10408417109104477. [DOI] [PubMed] [Google Scholar]
  5. Pato M. L. Regulation of chromosome replication and the bacterial cell cycle. Annu Rev Microbiol. 1972;26:347–368. doi: 10.1146/annurev.mi.26.100172.002023. [DOI] [PubMed] [Google Scholar]
  6. Reeve J. N., Clark D. J. Cell division of Escherichia coli BUG-6: effect of varying the length of growth at the nonpermissive temperature. J Bacteriol. 1972 Apr;110(1):117–121. doi: 10.1128/jb.110.1.117-121.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Reeve J. N., Clark D. J. Cell division of Escherichia coli BUG-6: effect of varying the temperature used as the nonpermissive growth condition. J Bacteriol. 1972 Apr;110(1):122–125. doi: 10.1128/jb.110.1.122-125.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Reeve J. N., Groves D. J., Clark D. J. Regulation of Cell Division in Escherichia coli: Characterization of Temperature-Sensitive Division Mutants. J Bacteriol. 1970 Dec;104(3):1052–1064. doi: 10.1128/jb.104.3.1052-1064.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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