Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Oct;116(1):257–262. doi: 10.1128/jb.116.1.257-262.1973

Effects of External Osmolarity on Phospholipid Metabolism in Escherichia coli B1

George F Munro a, Carol A Bell b
PMCID: PMC246416  PMID: 4583214

Abstract

The turnover of total [32P]phospholipids in Escherichia coli B is shown to be inversely related to the osmolarity of the medium and a reflection of the rates of turnover of the major phospholipid classes, phosphatidylglycerol and phosphatidylethanolamine. External osmolarity also affects the phosphatidylglycerol content of the culture. These results suggest that alterations in the metabolism of membrane phospholipids may be part of the process of adaptation to the external osmotic environment.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. Ballesta J. P., Schaechter M. Effect of shift-down and growth inhibition on phospholipid metabolism of Escherichia coli. J Bacteriol. 1971 Jul;107(1):251–258. doi: 10.1128/jb.107.1.251-258.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cronan J. E., Jr Phospholipid alterations during growth of Escherichia coli. J Bacteriol. 1968 Jun;95(6):2054–2061. doi: 10.1128/jb.95.6.2054-2061.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cronan J. E., Vagelos P. R. Metabolism and function of the membrane phospholipids of Escherichia coli. Biochim Biophys Acta. 1972 Feb 14;265(1):25–60. doi: 10.1016/0304-4157(72)90018-4. [DOI] [PubMed] [Google Scholar]
  6. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furrow M. H., Pizer L. I. Phospholipid synthesis in Escherichia coli infected with T4 bacteriophages. J Virol. 1968 Jun;2(6):594–605. doi: 10.1128/jvi.2.6.594-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Golden N. G., Powell G. L. Stringent and relaxed control of phospholipid metabolism in Escherichia coli. J Biol Chem. 1972 Oct 25;247(20):6651–6658. [PubMed] [Google Scholar]
  10. Inouye M. Reversal by sodium chloride of envelope protein changes related to DNA replication and cell division of Escherichia coli. J Mol Biol. 1972 Feb 14;63(3):597–600. doi: 10.1016/0022-2836(72)90451-2. [DOI] [PubMed] [Google Scholar]
  11. KANFER J., KENNEDY E. P. METABOLISM AND FUNCTION OF BACTERIAL LIPIDS. I. METABOLISM OF PHOSPHOLIPIDS IN ESCHERICHIA COLI B. J Biol Chem. 1963 Sep;238:2919–2922. [PubMed] [Google Scholar]
  12. Kanemasa Y., Akamatsu Y., Nojima S. Composition and turnover of the phospholipids in Escherichia coli. Biochim Biophys Acta. 1967 Oct 2;144(2):382–390. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Lennarz W. J. Lipid metabolism in the bacteria. Adv Lipid Res. 1966;4:175–225. doi: 10.1016/b978-1-4831-9940-5.50012-0. [DOI] [PubMed] [Google Scholar]
  15. Munro G. F., Hercules K., Morgan J., Sauerbier W. Dependence of the putrescine content of Escherichia coli on the osmotic strength of the medium. J Biol Chem. 1972 Feb 25;247(4):1272–1280. [PubMed] [Google Scholar]
  16. NICHOLS B. W. SEPARATION OF THE LIPIDS OF PHOTOSYNTHETIC TISSUES: IMPROVEMENTS IN ANALYSIS BY THIN-LAYER CHROMATOGRAPHY. Biochim Biophys Acta. 1963 Aug 27;70:417–422. doi: 10.1016/0006-3002(63)90771-6. [DOI] [PubMed] [Google Scholar]
  17. Nagata Y., Shibuya I., Maruo B. Preparation and properties of an active membrane system from Escherichia coli. J Biochem. 1967 May;61(5):623–632. doi: 10.1093/oxfordjournals.jbchem.a128592. [DOI] [PubMed] [Google Scholar]
  18. Okuyama H. Phospholipid metabolism in Escherichia coli after a shift in temperature. Biochim Biophys Acta. 1969 Jan 21;176(1):125–134. [PubMed] [Google Scholar]
  19. Randle C. L., Albro P. W., Dittmer J. C. The phosphoglyceride composition of Gram-negative bacteria and the changes in composition during growth. Biochim Biophys Acta. 1969;187(2):214–220. doi: 10.1016/0005-2760(69)90030-7. [DOI] [PubMed] [Google Scholar]
  20. Ricard M., Hirota Y. Effet des sels sur le processus de division cellulaire d'E. coli. C R Acad Sci Hebd Seances Acad Sci D. 1969 Mar 3;268(9):1335–1338. [PubMed] [Google Scholar]
  21. Siccardi A. G., Shapiro B. M. On the process of cellular division in Escherichia coli. IV. Altered protein composition and turnover of the membranes of thermosensitive mutants defective in chromosomal replication. J Mol Biol. 1971 Mar 28;56(3):475–490. doi: 10.1016/0022-2836(71)90395-0. [DOI] [PubMed] [Google Scholar]
  22. White D. C., Tucker A. N. Phospholipid metabolism during bacterial growth. J Lipid Res. 1969 Mar;10(2):220–233. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES