Abstract
Procedures are described for obtaining efficient mating (zygote formation) in the heterothallic yeast Saccharomyces cerevisiae. The methods separate operationally the initial, mating-specific aggregation from subsequent steps in conjugation. This cell-cell interaction has been characterized. The data support the conclusions that (i) aggregation in liquid suspension is a random collision process, and (ii) only non-budded cells participate in aggregation. A mathematical model for the kinetics of aggregation in liquid suspension has been developed which is in good agreement with the experimental data.
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- Bücking-Throm E., Duntze W., Hartwell L. H., Manney T. R. Reversible arrest of haploid yeast cells in the initiation of DNA synthesis by a diffusible sex factor. Exp Cell Res. 1973 Jan;76(1):99–110. doi: 10.1016/0014-4827(73)90424-2. [DOI] [PubMed] [Google Scholar]
- Crandall M. A., Brock T. D. Molecular basis of mating in the yeast hansenula wingei. Bacteriol Rev. 1968 Sep;32(3):139–163. doi: 10.1128/br.32.3.139-163.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duntze W., MacKay V., Manney T. R. Saccharomyces cerevisiae: a diffusible sex factor. Science. 1970 Jun 19;168(3938):1472–1473. doi: 10.1126/science.168.3938.1472. [DOI] [PubMed] [Google Scholar]
- Hartwell L. H. Synchronization of haploid yeast cell cycles, a prelude to conjugation. Exp Cell Res. 1973 Jan;76(1):111–117. doi: 10.1016/0014-4827(73)90425-4. [DOI] [PubMed] [Google Scholar]
- Hawthorne D. C., Mortimer R. K. Genetic mapping of nonsense suppressors in yeast. Genetics. 1968 Dec;60(4):735–742. doi: 10.1093/genetics/60.4.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STUART D. C., Jr Fine structure of the nucleoid and internal membrane systems of Streptomyces. J Bacteriol. 1959 Aug;78:272–281. doi: 10.1128/jb.78.2.272-281.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakai K., Yanagishima N. Mating reaction in Saccharomyces cerevisiae. I. Cell agglutination related to mating. Arch Mikrobiol. 1971;75(3):260–265. doi: 10.1007/BF00408986. [DOI] [PubMed] [Google Scholar]
- Sakai K., Yanagishima N. Mating reaction in Saccharomyces cerevisiae. II. Hormonal regulation of agglutinability of a type cells. Arch Mikrobiol. 1972;84(3):191–198. doi: 10.1007/BF00425197. [DOI] [PubMed] [Google Scholar]
- Sena E. P., Radin D. N., Fogel S. Synchronous mating in yeast. Proc Natl Acad Sci U S A. 1973 May;70(5):1373–1377. doi: 10.1073/pnas.70.5.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Throm E., Duntze W. Mating-Type-Dependent Inhibition of Deoxyribonucleic Acid Synthesis in Saccharomyces cerevisiae. J Bacteriol. 1970 Dec;104(3):1388–1390. doi: 10.1128/jb.104.3.1388-1390.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]