Abstract
The kinetics of reaction of the exposed ribosomal proteins from Escherichia coli toward N-ethylmaleimide (NEM) have been studied. While most of the proteins from the 30S subunit react rapidly with NEM and plateau levels can be reached, most of the proteins from the 50S subunit show biphasic uptake kinetics. Furthermore, differences have been observed in the reactivity of proteins toward NEM depending on whether the protein is in the free subunit or in the 70S ribosome.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chang F. N., Flaks J. G. Topography of the Escherichia coli 30S ribosomal subunit and streptomycin binding. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1321–1328. doi: 10.1073/pnas.67.3.1321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hardy S. J., Kurland C. G., Voynow P., Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969 Jul;8(7):2897–2905. doi: 10.1021/bi00835a031. [DOI] [PubMed] [Google Scholar]
- Huang K., Cantor C. R. Surface topography of the 30 s Escherichia coli ribosomal subunit: reactivity towards fluorescein isothiocyanate. J Mol Biol. 1972 Jun 20;67(2):265–275. doi: 10.1016/0022-2836(72)90240-9. [DOI] [PubMed] [Google Scholar]
- Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
- Moore P. B. Reaction of N-ethyl maleimide with the ribosomes of Escherichia coli. J Mol Biol. 1971 Aug 28;60(1):169–184. doi: 10.1016/0022-2836(71)90456-6. [DOI] [PubMed] [Google Scholar]
- NIRENBERG M. W., MATTHAEI J. H. The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Proc Natl Acad Sci U S A. 1961 Oct 15;47:1588–1602. doi: 10.1073/pnas.47.10.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nashimoto H., Held W., Kaltschmidt E., Nomura M. Structure and function of bacterial ribosomes. XII. Accumulation of 21 s particles by some cold-sensitive mutants of Escherichia coli. J Mol Biol. 1971 Nov 28;62(1):121–138. doi: 10.1016/0022-2836(71)90135-5. [DOI] [PubMed] [Google Scholar]
- Slobin L. I. Sulfhydryl groups of Escherichia coli Ribosomes and ribosomal proteins. J Mol Biol. 1971 Oct 14;61(1):281–285. doi: 10.1016/0022-2836(71)90227-0. [DOI] [PubMed] [Google Scholar]
- Staehelin T., Maglott D. M., Monro R. E. On the catalytic center of peptidyl transfer: a part of the 50 S ribosome structure. Cold Spring Harb Symp Quant Biol. 1969;34:39–48. doi: 10.1101/sqb.1969.034.01.008. [DOI] [PubMed] [Google Scholar]