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. 1973 Dec;116(3):1100–1106. doi: 10.1128/jb.116.3.1100-1106.1973

Gene Transmission Among Strains of Erwinia amylovora

Arun K Chatterjee 1, Mortimer P Starr 1
PMCID: PMC246462  PMID: 4752937

Abstract

Stable donor strains of Erwinia amylovora were obtained from strain EA178R1 (harboring an Escherichia coli F′lac) by selection for clones resistant to curing by acridine orange. These donor strains (EA178R1-99 and EA178R1-111) transfer chromosomal markers (arg, cys, gua, ilv, met, pro, ser, trp); the frequency of the appearance of recombinants prototrophic for Cys, Gua, Met, Ser, and Trp is highest (> 10−5), followed by recombinants prototrophic for Arg, Ilv, and Pro (10−7 to 10−5). The results of interrupted matings, as well as the frequency of transmission of various markers, suggest that cys is transferred as an early marker by both donor strains. The Hfr state of these donor strains is rather likely on the basis of the following observations. The donor strains exhibit a relatively efficient and possibly oriented chromosome transfer; the Lac+ character is not cured by acridine orange in these donor strains; and these donor strains do not transfer F.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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