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. 1973 Dec;116(3):1107–1112. doi: 10.1128/jb.116.3.1107-1112.1973

Isolation of Hfr Strains from R+ and ColV2+ Strains of Escherichia coli and Derivation of an R′lac Factor by Transduction

Akiko Nishimura 1, Yukinobu Nishimura 1, Lucien Caro 1
PMCID: PMC246463  PMID: 4584797

Abstract

Temperature-resistant revertants were isolated from Escherichia coli strains carrying a temperature-sensitive dnaA mutation (initiation of chromosome replication) and either a repressed or a derepressed F-like R factor or a ColV2 factor. Many of the revertants had all the properties of Hfr strains, with a variety of directions and origins of transfer. From one such revertant, episomes carrying the R factor and part of the lac region (R′lac) could be isolated by transduction. This system offers a good selection for Hfr strains produced by integration of various episomes and for the isolation of R′ factors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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