Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Dec;116(3):1177–1180. doi: 10.1128/jb.116.3.1177-1180.1973

Transformation and Physical Properties of R-Factor RP4 Transferred from Escherichia coli to Rhizobium trifolii

F O'Gara 1, L K Dunican 1
PMCID: PMC246472  PMID: 4584801

Abstract

Transformation of R-factor RP4 specifying resistance to ampicillin, kanamycin, and tetracycline from Escherichia coli to Rhizobium trifolii is reported. Partially purified RP4 deoxyribonucleic acid (DNA) of the donor strain E. coli J5-3 that carried the R-factor was prepared by the lysozyme-ethylenediaminetetraacetic acid-Triton X-100 procedure and was used in transformation experiments with R. trifolii as recipient. The frequency of transformation of the R-factor into R. trifolii was 1.3 × 10−4. Dye buoyant density and sucrose gradient centrifugation of R. trifolii DNA showed that the expression of the specified drug resistance of RP4 by R. trifolii was accompanied by the acquisition of an extrachromosomal, satellite DNA component which has indistinguishable physical properties from the R-factor in the donor strain. The significance of the transformation is discussed.

Full text

PDF
1177

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALASSA R. Durch Desoxyribonukleinsäuren induzierte Veränderungen bei Rhizobien. Acta Microbiol Acad Sci Hung. 1957;4(1):77–84. [PubMed] [Google Scholar]
  2. Balassa G. GENETIC TRANSFORMATION OF RHIZOBIUM: A REVIEW OF THE WORK OF R. BALASSA. Bacteriol Rev. 1963 Jun;27(2):228–241. doi: 10.1128/br.27.2.228-241.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gibbins A. M., Gregory K. F. Relatedness among Rhizobium and Agrobacterium species determined by three methods of nucleic acid hybridization. J Bacteriol. 1972 Jul;111(1):129–141. doi: 10.1128/jb.111.1.129-141.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kern H. Interspezifische Transformationen zwischen Agrobacterium tumefaciens und Rhizobium leguminosarum. Arch Mikrobiol. 1969;66(1):63–68. doi: 10.1007/BF00414664. [DOI] [PubMed] [Google Scholar]
  8. Klesius P. H., Schuhardt V. T. Use of lysostaphin in the isolation of highly polymerized deoxyribonucleic acid and in the taxonomy of aerobic Micrococcaceae. J Bacteriol. 1968 Mar;95(3):739–743. doi: 10.1128/jb.95.3.739-743.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nordström K., Ingram L. C., Lundbäck A. Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome. J Bacteriol. 1972 May;110(2):562–569. doi: 10.1128/jb.110.2.562-569.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rohrmann G. F., Krueger R. G. Physical, biochemical, and immunological properties of coliphage MS-2 particles. J Virol. 1970 Sep;6(3):269–279. doi: 10.1128/jvi.6.3.269-279.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roth T. F., Hayashi M. Allomorphic forms of bacteriophage phiX-174 replicative DNA. Science. 1966 Nov 4;154(3749):658–660. doi: 10.1126/science.154.3749.658. [DOI] [PubMed] [Google Scholar]
  12. SCHWINGHAMER E. A. Studies on induced variation in the rhizobia. I. Defined media and nodulation test techniques. Appl Microbiol. 1960 Nov;8:349–352. doi: 10.1128/am.8.6.349-352.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  14. Saunders J. R., Grinsted J. Properties of RP4, an R factor which originated in Pseudomonas aeruginosa S8. J Bacteriol. 1972 Nov;112(2):690–696. doi: 10.1128/jb.112.2.690-696.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vinograd J., Lebowitz J., Radloff R., Watson R., Laipis P. The twisted circular form of polyoma viral DNA. Proc Natl Acad Sci U S A. 1965 May;53(5):1104–1111. doi: 10.1073/pnas.53.5.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES