Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1973 Dec;116(3):1405–1411. doi: 10.1128/jb.116.3.1405-1411.1973

Electron Microscopy of the Carboxysomes (Polyhedral Bodies) of Thiobacillus neapolitanus

J M Shively 1, Frances L Ball 1, Betty W Kline 1
PMCID: PMC246500  PMID: 4127632

Abstract

The carboxysomes of Thiobacillus neapolitanus are shown, by electron microscopy, to consist of a paracrystalline array of 10-nm particles surrounded by a “membrane.” The 10-nm particles have a center hole or depression and have been previously identified as ribulose diphosphate carboxylase. The membrane is a monolayer approximately 3.5-nm thick.

Full text

PDF
1405

Images in this article

1406Image
on p.1406
1407Image
on p.1407
1409Image
on p.1409
1410Image
on p.1410

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akazawa T., Kondo H., Shimazue T., Nishimura M., Sugiyama T. Further studies on ribulose 1,5-diphosphate carboxylase from Chromatium strain D. Biochemistry. 1972 Mar 28;11(7):1298–1303. doi: 10.1021/bi00757a028. [DOI] [PubMed] [Google Scholar]
  2. Bowen C. C., Jensen T. E. Blue-Green Algae: Fine Structure of the Gas Vacuoles. Science. 1965 Mar 19;147(3664):1460–1462. doi: 10.1126/science.147.3664.1460. [DOI] [PubMed] [Google Scholar]
  3. Gantt E., Conti S. F. Ultrastructure of blue-green algae. J Bacteriol. 1969 Mar;97(3):1486–1493. doi: 10.1128/jb.97.3.1486-1493.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Holdsworth R. H. The isolation and partial characterization of the pyrenoid protein of Eremosphaera viridis. J Cell Biol. 1971 Nov;51(21):499–513. doi: 10.1083/jcb.51.2.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones D. D., Jost M. Isolation and chemical characterization of gas-vacuole membranes from Microcystis aeruginosa Kuetz. emend. Elenkin. Arch Mikrobiol. 1970;70(1):43–64. doi: 10.1007/BF00691059. [DOI] [PubMed] [Google Scholar]
  6. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MURRAY R. G., WATSON S. W. STRUCTURE OF NITROSOCYSTIS OCEANUS AND COMPARISON WITH NITROSOMONAS AND NITROBACTER. J Bacteriol. 1965 Jun;89:1594–1609. doi: 10.1128/jb.89.6.1594-1609.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MacElroy R. D., Johnson E. J., Johnson M. K. Characterization of ribulose diphosphate carboxylase and phosphoribulokinase from Thiobacillus thioparus and Thiobacillus neapolitanus. Arch Biochem Biophys. 1968 Sep 20;127(1):310–316. doi: 10.1016/0003-9861(68)90231-2. [DOI] [PubMed] [Google Scholar]
  9. McFadden B. A., Denend A. R. Ribulose diphosphate carboxylase from autotrophic microorganisms. J Bacteriol. 1972 May;110(2):633–642. doi: 10.1128/jb.110.2.633-642.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nicolson G. L., Schmidt G. L. Structure of the Chromatium sulfur particle and its protein membrane. J Bacteriol. 1971 Mar;105(3):1142–1148. doi: 10.1128/jb.105.3.1142-1148.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  12. Schmidt G. L., Nicolson G. L., Kamemd Composition of the sulfur particle of Chromatium vinosum strain D. J Bacteriol. 1971 Mar;105(3):1137–1141. doi: 10.1128/jb.105.3.1137-1141.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shively J. M., Ball F., Brown D. H., Saunders R. E. Functional organelles in prokaryotes: polyhedral inclusions (carboxysomes) of Thiobacillus neapolitanus. Science. 1973 Nov 9;182(4112):584–586. doi: 10.1126/science.182.4112.584. [DOI] [PubMed] [Google Scholar]
  14. Shively J. M., Decker G. L., Greenawalt J. W. Comparative ultrastructure of the thiobacilli. J Bacteriol. 1970 Feb;101(2):618–627. doi: 10.1128/jb.101.2.618-627.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wolk C. P. Movement of carbon from vegetative cells to heterocysts in Anabaena cylindrica. J Bacteriol. 1968 Dec;96(6):2138–2143. doi: 10.1128/jb.96.6.2138-2143.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolk C. P. Physiology and cytological chemistry blue-green algae. Bacteriol Rev. 1973 Mar;37(1):32–101. doi: 10.1128/br.37.1.32-101.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES