Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Jan;117(1):283–289. doi: 10.1128/jb.117.1.283-289.1974

Characterization of Three Plasmid Deoxyribonucleic Acid Molecules in a Strain of Streptococcus faecalis: Identification of a Plasmid Determining Erythromycin Resistance

D B Clewell 1, Y Yagi 1, G M Dunny 1, S K Schultz 1
PMCID: PMC246555  PMID: 4202995

Abstract

Three plasmids designated α, β, and γ, distinguishable by their molecular weights (6, 17, and 34 million, respectively) were isolated from Streptococcus faecalis strain DS-5 (ATCC 14508). Derivatives of this strain “cured” for erythromycin resistance lacked the β-plasmid. In the parent strain the β-plasmid was estimated to be present to the extent of one to two copies per chromosomal genome equivalent whereas the α- and γ-plasmids were about nine and five copies, respectively.

Full text

PDF
283

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bak A. L., Christiansen C., Stenderup A. Bacterial genome sizes determined by DNA renaturation studies. J Gen Microbiol. 1970 Dec;64(3):377–380. doi: 10.1099/00221287-64-3-377. [DOI] [PubMed] [Google Scholar]
  2. Bazaral M., Helinski D. R. Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513–3520. doi: 10.1021/bi00850a028. [DOI] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Courvalin P. M., Carlier C., Chabbert Y. A. Plasmid-linked tetracycline and erythromycin resistance in group D "streptococcus". Ann Inst Pasteur (Paris) 1972 Dec;123(6):755–759. [PubMed] [Google Scholar]
  6. Dunny G. M., Birch N., Hascall G., Clewell D. B. Isolation and characterization of plasmid deoxyribonucleic acid from Streptococcus mutans. J Bacteriol. 1973 Jun;114(3):1362–1364. doi: 10.1128/jb.114.3.1362-1364.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MACHATTIE L. A., BERNE K. I., THOMAS C. A., Jr ELECTRON MICROSCOPY OF DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:648–649. doi: 10.1016/s0022-2836(65)80019-5. [DOI] [PubMed] [Google Scholar]
  8. Malke H. Resistance pattern and genetics of erythromycin-resistant mutants of Streptococcus pyogenes. J Gen Microbiol. 1970 Dec;64(3):353–363. doi: 10.1099/00221287-64-3-353. [DOI] [PubMed] [Google Scholar]
  9. Toala P., McDonald A., Wilcox C., Finland M. Susceptibility of group D streptococcus (enterococcus) to 21 antibiotics in vitro, with special reference to species differences. Am J Med Sci. 1969 Dec;258(6):416–430. doi: 10.1097/00000441-196912000-00006. [DOI] [PubMed] [Google Scholar]
  10. Weisblum B., Demohn V. Erythromycin-inducible resistance in Staphylococcus aureus: survey of antibiotic classes involved. J Bacteriol. 1969 May;98(2):447–452. doi: 10.1128/jb.98.2.447-452.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES