Abstract
Certain classes of pyocyanine mutants in Pseudomonas aeruginosa escape detection when screened in the presence of wild-type or other mutant cells. A technique is described for recognizing mutant phenotypes after cells are in individual agar wells. The procedure eliminates cross-feeding phenomena as well as the masking of mutant phenotypes by diffusing pyocyanine produced by nearby clones.
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Selected References
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- Calhoun D. H., Carson M., Jensen R. A. The branch point metabolite for pyocyanine biosynthesis in Pseudomonas aeruginosa. J Gen Microbiol. 1972 Oct;72(3):581–583. doi: 10.1099/00221287-72-3-581. [DOI] [PubMed] [Google Scholar]
- KING E. O., WARD M. K., RANEY D. E. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed] [Google Scholar]
- Longley R. P., Halliwell J. E., Campbell J. J., Ingledew W. M. The branchpoint of pyocyanine biosynthesis. Can J Microbiol. 1972 Sep;18(9):1357–1363. doi: 10.1139/m72-210. [DOI] [PubMed] [Google Scholar]