Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Mar;117(3):994–1001. doi: 10.1128/jb.117.3.994-1001.1974

Reversion of Frameshift Mutations by Mutator Genes in Escherichia coli

Eli C Siegel 1, Freja Kamel 1
PMCID: PMC246577  PMID: 4360543

Abstract

The Escherichia coli mutator genes mutU4, mutS3, and mut-25 (a possible allele of mutL), previously known to induce transitional base changes, increased significantly the frequencies of reversion of lacZ frameshift mutations. mutT1, previously shown to induce only the transversion of adenine-thymine to cytosine-guanine, had no effect on the reversion of lacZ frameshift mutations. With mutator genes other than mutT1, small increases were found in the frequencies of reversion of trpA frameshift mutations.

Full text

PDF
994

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins J. F., Elseviers D., Gorini L. Low activity of -galactosidase in frameshift mutants of Escherichia coli. Proc Natl Acad Sci U S A. 1972 May;69(5):1192–1195. doi: 10.1073/pnas.69.5.1192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BECKWITH J. R. A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI. J Mol Biol. 1964 Mar;8:427–430. doi: 10.1016/s0022-2836(64)80206-0. [DOI] [PubMed] [Google Scholar]
  3. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berger H., Brammar W. J., Yanofsky C. Analysis of amino acid replacements resulting from frameshift and missense mutations in the tryptophan synthetase A gene of Escherichia coli. J Mol Biol. 1968 Jul 14;34(2):219–238. doi: 10.1016/0022-2836(68)90248-9. [DOI] [PubMed] [Google Scholar]
  5. Berger H., Brammar W. J., Yanofsky C. Spontaneous and ICR191-A-induced frameshift mutations in the A gene of Escherichia coli tryptophan synthetase. J Bacteriol. 1968 Nov;96(5):1672–1679. doi: 10.1128/jb.96.5.1672-1679.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brammar W. J. Independent expression of the A gene of the tryptophan operon of Escherichia coli during tryptophan starvation. J Gen Microbiol. 1973 Jun;76(2):395–405. doi: 10.1099/00221287-76-2-395. [DOI] [PubMed] [Google Scholar]
  7. Breckenridge L., Gorini L. Genetic analysis of streptomycin resistance in Escherichia coli. Genetics. 1970 May;65(1):9–25. doi: 10.1093/genetics/65.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cox E. C., Degnen G. E., Scheppe M. L. Mutator gene studies in Escherichia coli: the mutS gene. Genetics. 1972 Dec;72(4):551–567. doi: 10.1093/genetics/72.4.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hill R. F. Location of genes controlling excision repair of UV damage and mutator activity in Escherichia coli WP2. Mutat Res. 1970 Mar;9(3):341–344. doi: 10.1016/0027-5107(70)90135-1. [DOI] [PubMed] [Google Scholar]
  10. Imada M., Inouye M., Eda M., Tsugita A. Frameshift mutation in the lysozyme gene of bacteriophage T4: demonstration of the insertion offour bases and the preferential occurrence of base addition in acridine mutagenesis. J Mol Biol. 1970 Dec 14;54(2):199–217. doi: 10.1016/0022-2836(70)90427-4. [DOI] [PubMed] [Google Scholar]
  11. Liberfarb R. M., Bryson V. Isolation, characterization, and genetic analysis of mutator genes in Escherichia coli B and K-12. J Bacteriol. 1970 Oct;104(1):363–375. doi: 10.1128/jb.104.1.363-375.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Luria S. E., Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. doi: 10.1093/genetics/28.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Newton A. Isolation and characterization of frameshift mutations in the lac operon. J Mol Biol. 1970 May 14;49(3):589–601. doi: 10.1016/0022-2836(70)90283-4. [DOI] [PubMed] [Google Scholar]
  14. Newton W. A., Beckwith J. R., Zipser D., Brenner S. Nonsense mutants and polarity in the lac operon of Escherichia coli. J Mol Biol. 1965 Nov;14(1):290–296. doi: 10.1016/s0022-2836(65)80250-9. [DOI] [PubMed] [Google Scholar]
  15. Oeschger N. S., Hartman P. E. ICR-induced frameshift mutations in the histidine operon of Salmonella. J Bacteriol. 1970 Feb;101(2):490–504. doi: 10.1128/jb.101.2.490-504.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Riddle D. L., Roth J. R. Frameshift suppressors. 3. Effects of suppressor mutations on transfer RNA. J Mol Biol. 1972 May 28;66(3):495–506. doi: 10.1016/0022-2836(72)90429-9. [DOI] [PubMed] [Google Scholar]
  17. Scaife J., Beckwith J. R. Mutational alteration of the maximal level of Lac operon expression. Cold Spring Harb Symp Quant Biol. 1966;31:403–408. doi: 10.1101/sqb.1966.031.01.052. [DOI] [PubMed] [Google Scholar]
  18. Siegel E. C., Bryson V. Mutator gene of Escherichia coli B. J Bacteriol. 1967 Jul;94(1):38–47. doi: 10.1128/jb.94.1.38-47.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Siegel E. C. Ultraviolet-sensitive mutator mutU4 of Escherichia coli inviable with polA. J Bacteriol. 1973 Jan;113(1):161–166. doi: 10.1128/jb.113.1.161-166.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Siegel E. C. Ultraviolet-sensitive mutator strain of Escherichia coli K-12. J Bacteriol. 1973 Jan;113(1):145–160. doi: 10.1128/jb.113.1.145-160.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Silengo L., Schlessinger D., Mangiarotti G., Apirion D. Induction of mutations to streptomycin and spectinomycin resistance in Escherichia coli by N-methyl-N'-nitroso-N-nitroguanidine and acridine half-mustard ICR-191. Mutat Res. 1967 Sep-Oct;4(5):701–703. doi: 10.1016/0027-5107(67)90056-5. [DOI] [PubMed] [Google Scholar]
  22. Smirnov G. B., Skavronskaya A. G. Location of uvr502 mutation on the chromosome of Escherichia coli K-12. Mol Gen Genet. 1971;113(3):217–221. doi: 10.1007/BF00339541. [DOI] [PubMed] [Google Scholar]
  23. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Treffers H. P., Spinelli V., Belser N. O. A Factor (or Mutator Gene) Influencing Mutation Rates in Escherichia Coli. Proc Natl Acad Sci U S A. 1954 Nov;40(11):1064–1071. doi: 10.1073/pnas.40.11.1064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yanofsky C., Cox E. C., Horn V. The unusual mutagenic specificity of an E. Coli mutator gene. Proc Natl Acad Sci U S A. 1966 Feb;55(2):274–281. doi: 10.1073/pnas.55.2.274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. van de Putte P., van Sluis C. A., van Dillewijn J., Rörsch A. The location of genes controlling radiation sensitivity in Escherichia coli. Mutat Res. 1965 Apr;2(2):97–110. doi: 10.1016/0027-5107(65)90041-2. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES