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. 1974 Mar;117(3):1093–1098. doi: 10.1128/jb.117.3.1093-1098.1974

Sodium-Stimulated Glutamate Transport in Osmotically Shocked Cells and Membrane Vesicles of Escherichia coli

Karen M Miner 1, Leonard Frank 1
PMCID: PMC246588  PMID: 4591944

Abstract

Three phenotypically distinct strains of Escherichia coli B were studied: one in which the transport of glutamate was strongly stimulated by sodium, one in which the transport was relatively independent of sodium, and one which did not transport glutamate. Membrane vesicle preparations from the three strains followed the behavior of whole cells with respect to sodium-stimulated transport. Although glutamate-binding material could be released from cells by osmotic shock, its affinity for glutamate was not significantly influenced by sodium. Furthermore, the shocked cells retained sodium-stimulated transport. The accumulated results suggest that the sodium-activated glutamate transport system resides in the cytoplasmic membrane and that releasable binding protein(s) is not intimately involved in its function.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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