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. 1974 Mar;117(3):1117–1120. doi: 10.1128/jb.117.3.1117-1120.1974

Isolation and Characterization of Low-Kynureninase Mutants of Neurospora crassa

Steven C Schlitt a, Gabriel Lester a,1, Peter J Russell a
PMCID: PMC246591  PMID: 4273316

Abstract

Two kynureninase activities are known in Neurospora crassa, one of which (kynureninase I) is inducible, the other (kynureninase II) being constitutive. A method is described for the isolation of low-kynureninase mutants of N. crassa. When grown on an inducer, the mutants show significantly less kynureninase I activity compared with wild type, whereas constitutive kynureninase II activity is unaffected. Since a low level of kynureninase I activity remains in the mutants examined, the mutations may be in a regulatory gene or genes. Other experiments are described concerning the molecular weights of the two enzymes and the intracellular localization and specificity of kynureninase II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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