Abstract
Cell walls made by Bacillus subtilis bacteria grown in D2O medium have buoyant densities in CsCl which are different from walls made by cells grown in H2O medium. Cell wall turnover was studied by measuring the change in wall buoyant density after a B. subtilis culture was shifted from growth in D2O medium to aeration in H2O medium. Walls from the hemispherical caps were isolated after preferential digestion of wall from the cylindrical regions using the B. subtilis autolytic amidase. The walls from the polar regions were found to turn over extensively.
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