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. 1974 Apr;118(1):8–14. doi: 10.1128/jb.118.1.8-14.1974

Carbohydrate Metabolism During Ascospore Development in Yeast

Sherwin M Kane 1, Robert Roth 1
PMCID: PMC246633  PMID: 4595206

Abstract

Carbohydrate metabolism, under sporulation conditions, was compared in sporulating and non-sporulating diploids of Saccharomyces cerevisiae. Total carbohydrate was fractionated into trehalose, glycogen, mannan, and an alkali-insoluble fraction composed of glucan and insoluble glycogen. The behavior of three fractions was essentially the same in both sporulating and non-sporulating strains; trehalose, mannan, and the insoluble fraction were all synthesized to about the same extent regardless of a strain's ability to undergo meiosis or sporulation. In contrast, aspects of soluble glycogen metabolism depended on sporulation. Although glycogen synthesis took place in both sporulating and non-sporulating strains, only sporulating strains exhibited a period of glycogen degradation, which coincided with the final maturation of ascospores. We also determined the carbohydrate composition of spores isolated from mature asci. Spores contained all components present in vegetative cells, but in different proportions. In cells, the most abundant carbohydrate was mannan, followed by glycogen, then trehalose, and finally the alkali-insoluble fraction; in spores, trehalose was most abundant, followed by the alkali-insoluble fraction, glycogen, and mannan in that order.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERKE H. L., ROTHSTEIN A. The metabolism of storage carbohydrates in yeast, studied with glucose-1-C14 and dinitrophenol. Arch Biochem Biophys. 1957 Dec;72(2):380–395. doi: 10.1016/0003-9861(57)90215-1. [DOI] [PubMed] [Google Scholar]
  2. Friis J., Roman H. The effect of the mating-type alleles on intragenic recombination in yeast. Genetics. 1968 May;59(1):33–36. doi: 10.1093/genetics/59.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Henry S. A., Halvorson H. O. Lipid synthesis during sporulation of Saccharomyces cerevisiae. J Bacteriol. 1973 Jun;114(3):1158–1163. doi: 10.1128/jb.114.3.1158-1163.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MOKRASCH L. C. Analysis of hexose phosphates and sugar mixtures with the anthrone reagent. J Biol Chem. 1954 May;208(1):55–59. [PubMed] [Google Scholar]
  5. NORTHCOTE D. H., HORNE R. W. The chemical composition and structure of the yeast cell wall. Biochem J. 1952 May;51(2):232–236. doi: 10.1042/bj0510232. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. PANEK A. Synthesis of trehalose by baker's yeast (Saccharomyces cerevisiae). Arch Biochem Biophys. 1962 Sep;98:349–355. doi: 10.1016/0003-9861(62)90197-2. [DOI] [PubMed] [Google Scholar]
  7. Roman H., Sands S. M. Heterogeneity of Clones of Saccharomyces Derived from Haploid Ascospores. Proc Natl Acad Sci U S A. 1953 Mar;39(3):171–179. doi: 10.1073/pnas.39.3.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Roth R. Carbohydrate accumulation during the sporulation of yeast. J Bacteriol. 1970 Jan;101(1):53–57. doi: 10.1128/jb.101.1.53-57.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Roth R., Halvorson H. O. Sporulation of yeast harvested during logarithmic growth. J Bacteriol. 1969 May;98(2):831–832. doi: 10.1128/jb.98.2.831-832.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roth R., Lusnak K. DNA synthesis during yeast sporulation: genetic control of an early developmental event. Science. 1970 Apr 24;168(3930):493–494. doi: 10.1126/science.168.3930.493. [DOI] [PubMed] [Google Scholar]
  11. Rothman L. B., Cabib E. Regulation of glycogen synthesis in the intact yeast cell. Biochemistry. 1969 Aug;8(8):3332–3341. doi: 10.1021/bi00836a030. [DOI] [PubMed] [Google Scholar]
  12. Rousseau P., Halvorson H. O. Preparation and storage of single spores of Saccharomyces cerevisiae. J Bacteriol. 1969 Dec;100(3):1426–1427. doi: 10.1128/jb.100.3.1426-1427.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TREVELYAN W. E., HARRISON J. S. Studies on yeast metabolism. 7. Yeast carbohydrate fractions. Separation from nucleic acid, analysis, and behaviour during anaerobic fermentation. Biochem J. 1956 May;63(1):23–33. doi: 10.1042/bj0630023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TREVELYAN W. E., HARRISON J. S. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J. 1952 Jan;50(3):298–303. doi: 10.1042/bj0500298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vezinhet F., Arnaud A., Galzy P. Etude de la respiration de la levure au cours de la sporulation. Can J Microbiol. 1971 Sep;17(9):1179–1184. [PubMed] [Google Scholar]

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