Abstract
Reversed-phase chromatography has been used to detect the presence of a new form of phenylalanyl-transfer ribonucleic acid (Phe-tRNA) from Escherichia coli growing exponentially in media containing low but nonlimiting levels of inorganic phosphate. The amount of this extra Phe-tRNA form is greatest in slowly growing cells (0.8 generations/h), and becomes negligible in media supporting a rapid growth rate (2.14 generations/h).
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barrell B. G., Sanger F. The sequence of phenylalanine tRNA from E. coli. FEBS Lett. 1969 Jun;3(4):275–278. doi: 10.1016/0014-5793(69)80157-2. [DOI] [PubMed] [Google Scholar]
- Bartz J., Söll D., Burrows W. J., Skoog F. Identification of the cytokinin-active ribonucleosides in pure Escherichia coli tRNA species. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1448–1453. doi: 10.1073/pnas.67.3.1448. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gefter M. L., Russell R. L. Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding. J Mol Biol. 1969 Jan 14;39(1):145–157. doi: 10.1016/0022-2836(69)90339-8. [DOI] [PubMed] [Google Scholar]
- Gross H. J., Raab C. In vivo synthesis of tRNA Tyr 1 and tRNA Tyr 2 : differences in "early" and "late log" E. coli MRE 600. Biochem Biophys Res Commun. 1972 Mar 24;46(6):2006–2011. doi: 10.1016/0006-291x(72)90751-6. [DOI] [PubMed] [Google Scholar]
- Littauer U. Z., Inouye H. Regulation of tRNA. Annu Rev Biochem. 1973;42:439–470. doi: 10.1146/annurev.bi.42.070173.002255. [DOI] [PubMed] [Google Scholar]
- Mann M. B., Huang P. C. Behavior of chloramphenicol-induced phenylalanine transfer ribonucleic acid during recovery from chloramphenicol treatment in Escherichia coli. Biochemistry. 1973 Dec 18;12(26):5289–5294. doi: 10.1021/bi00750a011. [DOI] [PubMed] [Google Scholar]
- Pearson R. L., Weiss J. F., Kelmers A. D. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns. Biochim Biophys Acta. 1971 Feb 11;228(3):770–774. doi: 10.1016/0005-2787(71)90748-9. [DOI] [PubMed] [Google Scholar]
- Rosenberg A. H., Gefter M. L. An iron-dependent modification of several transfer RNA species in Escherichia coli. J Mol Biol. 1969 Dec 28;46(3):581–584. doi: 10.1016/0022-2836(69)90197-1. [DOI] [PubMed] [Google Scholar]
- Waters L. C. Altered chromatographic properties of tRNA from chloramphenicol-treated Escherichia coli. Biochem Biophys Res Commun. 1969 Oct 8;37(2):296–304. doi: 10.1016/0006-291x(69)90734-7. [DOI] [PubMed] [Google Scholar]
- Waters L. C., Novelli G. D. A new change in leucine transfer RNA observed in Escherichia coli infected with bacteriophage T2. Proc Natl Acad Sci U S A. 1967 Apr;57(4):979–985. doi: 10.1073/pnas.57.4.979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wettstein F. O., Stent G. S. Physiologically induced changes in the property of phenylalanine tRNA in Escherichia coli. J Mol Biol. 1968 Nov 28;38(1):25–40. doi: 10.1016/0022-2836(68)90126-5. [DOI] [PubMed] [Google Scholar]
- Yang W. K., Novelli G. D. Isoaccepting +RNA's in mouse plasma cell tumors that synthesize different myeloma protein. Biochem Biophys Res Commun. 1968 May 23;31(4):534–539. doi: 10.1016/0006-291x(68)90510-x. [DOI] [PubMed] [Google Scholar]