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. 1974 May;118(2):329–333. doi: 10.1128/jb.118.2.329-333.1974

Localization of Proteinase(s) near the Cell Surface of Streptococcus lactis

T D Thomas a, B D W Jarvis a, N A Skipper a,1
PMCID: PMC246762  PMID: 4208129

Abstract

Two criteria suggest that most of the proteinase of Streptococcus lactis is localized in the cell wall. (i) Intact cells possess proteinase activity when incubated with a high-molecular-weight substrate. (ii) Most of the cell-bound proteinase activity is released during spheroplast formation under conditions which result in the release of only 1% of the intracellular enzymes aldolase and glyceraldehyde-3-phosphate dehydrogenase. The solubilized cell wall, plasma membrane, and cytoplasm fractions contained 84, 0, and 16%, respectively, of the total proteinase activity with casein as substrate. The physiological role of a surface-bound proteinase in this organism is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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