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. 1974 May;118(2):434–441. doi: 10.1128/jb.118.2.434-441.1974

An Unusual Mutant Affecting the Frequency of Organelle Mutation

Charles L Hershberger 1, Donna Morgan 1, Fred A Weaver 1, Phil Pienkos 1
PMCID: PMC246775  PMID: 4208133

Abstract

A mutant of Euglena gracilis Z is described that spontaneously produces irreversibly bleached mutants at a high frequency. Bleaching only occurs during exponential growth under highly aerobic conditions. The kinetics for bleaching and growth of bleached and nonbleached cells suggest that mutation is induced by segregation of a cellular component that becomes limiting after several culture generations. The properties of spontaneous bleaching closely resemble the properties of induced organelle mutagenesis in E. gracilis and yeast.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BUETOW D. E., PADILLA G. M. Growth of Astasia longo on ethanol. I. Effects of ethanol on generation time, population density and biochemical profile. J Protozool. 1963 Feb;10:121–123. doi: 10.1111/j.1550-7408.1963.tb01646.x. [DOI] [PubMed] [Google Scholar]
  3. Borst P. Mitochondrial nucleic acids. Annu Rev Biochem. 1972;41:333–376. doi: 10.1146/annurev.bi.41.070172.002001. [DOI] [PubMed] [Google Scholar]
  4. Ebringer L. The action of nalidixic acid on euglena plastids. J Gen Microbiol. 1970 Apr;61(1):141–144. doi: 10.1099/00221287-61-1-141. [DOI] [PubMed] [Google Scholar]
  5. GIBOR A., GRANICK S. PLASTIDS AND MITOCHONDRIA: INHERITABLE SYSTEMS. Science. 1964 Aug 14;145(3633):890–897. doi: 10.1126/science.145.3635.890. [DOI] [PubMed] [Google Scholar]
  6. Kempner E. S., Marr A. G. Growth in volume of Euglena gracilis during the division cycle. J Bacteriol. 1970 Feb;101(2):561–567. doi: 10.1128/jb.101.2.561-567.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kempner E. S., Miller J. H. The molecular biology of Euglena gracilis. VII. Inorganic requirements for a minimal culture medium. J Protozool. 1972 May;19(2):343–346. doi: 10.1111/j.1550-7408.1972.tb03472.x. [DOI] [PubMed] [Google Scholar]
  8. Luria S. E., Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. doi: 10.1093/genetics/28.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MARCOVICH H. Action de l'acriflavine sur les levures. VIII. Détermination du composant actif et étude de l'euflavine. Ann Inst Pasteur (Paris) 1951 Oct;81(4):452–468. [PubMed] [Google Scholar]
  10. MEGO J. L. THE EFFECT OF HADACIDIN ON CHLOROPLAST DEVELOPMENT IN NON-DIVIDING EUGLENA CELLS. Biochim Biophys Acta. 1964 Mar 30;79:221–225. [PubMed] [Google Scholar]
  11. Mego J. L., Buetow D. E. Influence of cell division on the degree of streptomycin bleaching of Euglena gracilis. J Protozool. 1966 Feb;13(1):20–23. doi: 10.1111/j.1550-7408.1966.tb01862.x. [DOI] [PubMed] [Google Scholar]
  12. RAUT C. Heritable non-genic changes induced in yeast by ultraviolet light. J Cell Physiol. 1954 Dec;44(3):463–475. doi: 10.1002/jcp.1030440308. [DOI] [PubMed] [Google Scholar]
  13. RYAN F. J., WAINWRIGHT L. K. Nuclear segregation and the growth of clones of spontaneous mutants of bacteria. J Gen Microbiol. 1954 Dec;11(3):364–379. doi: 10.1099/00221287-11-3-364. [DOI] [PubMed] [Google Scholar]
  14. SHERMAN F. The effects of elevated temperatures on yeast. II. Induction of respiratory-deficient mutants. J Cell Comp Physiol. 1959 Aug;54:37–52. doi: 10.1002/jcp.1030540106. [DOI] [PubMed] [Google Scholar]
  15. Weislogel P. O., Butow R. A. Control of the mitochondrial genome in Saccharomyces cerevisiae. The fate of mitochondrial membrane proteins and mitochondrial deoxyribonucleic acid during petite induction. J Biol Chem. 1971 Aug 25;246(16):5113–5119. [PubMed] [Google Scholar]

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