Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 May;118(2):523–533. doi: 10.1128/jb.118.2.523-533.1974

Effects of Oxygen Tension and Glucose Repression on Mitochondrial Protein Synthesis in Continuous Cultures of Saccharomyces cerevisiae

P J Rogers 1, S B Yue 1, P R Stewart 1
PMCID: PMC246785  PMID: 4275173

Abstract

Continuous cultures of Saccharomyces cerevisiae grown over a range of oxygen and glucose concentrations were used to determine the effects of these two physiological regulators on mitochondrial protein synthesis in vivo. Quantitative estimates were obtained of the contribution of the mitochondrial protein-synthesizing system to the formation of mitochondrial membranes in cells grown over a wide range of dissolved oxygen concentrations, under conditions of glucose limitation or glucose excess in the cultures. The nature of the products of the mitochondrial system formed under these conditions was examined by selectively labeling membrane proteins in vivo in the presence of cycloheximide, and fractionating the products by gel electrophoresis under dissociating conditions. These results have been correlated with changes in the lipid composition of the cells and with the synthesis and assembly of components of the mitochondrial adenosine 5′-triphosphatase complex.

Full text

PDF
523

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Duntze W., Neumann D., Gancedo J. M., Atzpodien W., Holzer H. Studies on the regulation and localization of the glyoxylate cycle enzymes in Saccharomyces cerevisiae. Eur J Biochem. 1969 Aug;10(1):83–89. doi: 10.1111/j.1432-1033.1969.tb00658.x. [DOI] [PubMed] [Google Scholar]
  2. Gordon P. A., Stewart P. R. Effect of lipid status on cytoplasmic and mitochondrial protein synthesis in anaerobic cultures of Saccharomyces cerevisiae. J Gen Microbiol. 1972 Sep;72(2):231–242. doi: 10.1099/00221287-72-2-231. [DOI] [PubMed] [Google Scholar]
  3. Gordon P. A., Syewart P. R. The effect of antibiotics on lipid synthesis during respiratory development in Saccharomyces cerevisiae. Microbios. 1971 Sep;4(14):115–132. [PubMed] [Google Scholar]
  4. Groot G. S., Rouslin W., Schatz G. Promitochondria of anaerobically grown yeast. VI. Effect of oxygen on promitochondrial protein synthesis. J Biol Chem. 1972 Mar 25;247(6):1735–1742. [PubMed] [Google Scholar]
  5. Johnson B., Nelson S. J., Brown C. M. Influence of glucose concentration on the physiology and lipid composition of some yeasts. Antonie Van Leeuwenhoek. 1972;38(2):129–136. doi: 10.1007/BF02328084. [DOI] [PubMed] [Google Scholar]
  6. Jollow D., Kellerman G. M., Linnane A. W. The biogenesis of mitochondria. 3. The lipid composition of aerobically and anaerobically grown Saccharomyces cerevisiae as related to the membrane systems of the cells. J Cell Biol. 1968 May;37(2):221–230. doi: 10.1083/jcb.37.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kormancíkov'A V., Kovác L., Vidová M. Oxidative phosphorylation in yeast. V. Phosphorylation efficiencies in growing cells determined from molar growth yields. Biochim Biophys Acta. 1969 May;180(1):9–17. doi: 10.1016/0005-2728(69)90188-1. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Polakis E. S., Bartley W. Changes in the enzyme activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochem J. 1965 Oct;97(1):284–297. doi: 10.1042/bj0970284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rogers P. J., Stewart P. R. Mitochondrial and peroxisomal contributions to the energy metabolism of Saccharomyces cerevisiae in continuous culture. J Gen Microbiol. 1973 Dec;79(2):205–217. doi: 10.1099/00221287-79-2-205. [DOI] [PubMed] [Google Scholar]
  11. Rogers P. J., Stewart P. R. Respiratory development in Saccharomyces cerevisiae grown at controlled oxygen tension. J Bacteriol. 1973 Jul;115(1):88–97. doi: 10.1128/jb.115.1.88-97.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schatz G. Impaired binding of mitochondrial adenosine triphosphatase in the cytoplasmic "petite" mutant of Saccharomyces cerevisiae. J Biol Chem. 1968 May 10;243(9):2192–2199. [PubMed] [Google Scholar]
  13. Schatz G., Saltzgaber J. Protein synthesis by yeast promitochondria in vivo. Biochem Biophys Res Commun. 1969 Dec 4;37(6):996–1001. doi: 10.1016/0006-291x(69)90230-7. [DOI] [PubMed] [Google Scholar]
  14. Szabo A. S., Avers C. J. Some aspects of regulation of peroxisomes and mitochondria in yeast. Ann N Y Acad Sci. 1969 Dec 19;168(2):302–312. doi: 10.1111/j.1749-6632.1969.tb43117.x. [DOI] [PubMed] [Google Scholar]
  15. Tzagoloff A. Assembly of the mitochondrial membrane system. IV. Role of mitochondrial and cytoplasmic protein synthesis in the biosynthesis of the rutamycin-sensitive adenosine triphosphatase. J Biol Chem. 1971 May 10;246(9):3050–3056. [PubMed] [Google Scholar]
  16. Tzagoloff A., Rubin M. S., Sierra M. F. Biosynthesis of mitochondrial enzymes. Biochim Biophys Acta. 1973 Feb 12;301(1):71–104. doi: 10.1016/0304-4173(73)90013-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES