Abstract
A series of Hfr deletion strains carrying deletions extending different distances into the integrated F factor have been used to map loci for surface exclusion (traS) and for incompatibility (inc) on the Escherichia coli K-12 sex factor F. traS mapped between traG and traD. It forms a part of the large operon, including all the known transfer genes except traJ, and is co-controlled with these. The product of traS is not required for formation of the F pilus. inc mapped between the φR11 locus and the origin of transfer; it is therefore one of the earliest loci transferred during conjugation.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Achtman M., Willetts N., Clark A. J. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli. J Bacteriol. 1972 Jun;110(3):831–842. doi: 10.1128/jb.110.3.831-842.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Alfaro G., Willetts N. The relationship between the transfer systems of some bacterial plasmids. Genet Res. 1972 Dec;20(3):279–289. doi: 10.1017/s0016672300013811. [DOI] [PubMed] [Google Scholar]
- Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bastarrachea F., Clark A. J. Isolation and characterization of an Escherichia coli strain harboring three sex factors. Genetics. 1968 Dec;60(4):641–660. doi: 10.1093/genetics/60.4.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Broda P., Meacock P., Achtman M. Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12. Mol Gen Genet. 1972;116(4):336–347. doi: 10.1007/BF00270090. [DOI] [PubMed] [Google Scholar]
- Clark A. J. The beginning of a genetic analysis of recombination proficiency. J Cell Physiol. 1967 Oct;70(2 Suppl):165–180. doi: 10.1002/jcp.1040700412. [DOI] [PubMed] [Google Scholar]
- DeVries J. K., Maas W. K. Chromosomal integration of F' factors in recombination-deficient Hfr strains of Escherichia coli. J Bacteriol. 1971 Apr;106(1):150–156. doi: 10.1128/jb.106.1.150-156.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeVries J. K., Maas W. K. Description of an incompatibility mutant of Escherichia coli. J Bacteriol. 1973 Jul;115(1):213–220. doi: 10.1128/jb.115.1.213-220.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubnau E., Maas W. K. Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli. J Bacteriol. 1968 Feb;95(2):531–539. doi: 10.1128/jb.95.2.531-539.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Echols H. PROPERTIES OF F' STRAINS OF ESCHERICHIA COLI SUPERINFECTED WITH F-LACTOSE AND F-GALATOSE EPISOMES. J Bacteriol. 1963 Feb;85(2):262–268. doi: 10.1128/jb.85.2.262-268.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finnegan D. J., Willetts N. S. Two classes of Flac mutants insensitive to transfer inhibition by an F-like R factor. Mol Gen Genet. 1971;111(3):256–264. doi: 10.1007/BF00433110. [DOI] [PubMed] [Google Scholar]
- Finnegan D., Willetts N. The site of action of the F transfer inhibitor. Mol Gen Genet. 1973 Dec 31;127(4):307–316. doi: 10.1007/BF00267101. [DOI] [PubMed] [Google Scholar]
- Freifelder D., Folkmanis A., Kirschner I. Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J Bacteriol. 1971 Mar;105(3):722–727. doi: 10.1128/jb.105.3.722-727.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hohn B., Korn D. Cosegregation of a sex factor with the Escherichia coli chromosome during curing by acridine orange. J Mol Biol. 1969 Oct 28;45(2):385–395. doi: 10.1016/0022-2836(69)90113-2. [DOI] [PubMed] [Google Scholar]
- Ippen-Ihler K., Achtman M., Willetts N. Deletion map of the Escherichia coli K-12 sex factor F: the order of eleven transfer cistrons. J Bacteriol. 1972 Jun;110(3):857–863. doi: 10.1128/jb.110.3.857-863.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lederberg J, Cavalli L L, Lederberg E M. Sex Compatibility in Escherichia Coli. Genetics. 1952 Nov;37(6):720–730. doi: 10.1093/genetics/37.6.720. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maas W. K., Goldschmidt A. D. A mutant of Escherichia coli permitting replication of two F factors. Proc Natl Acad Sci U S A. 1969 Mar;62(3):873–880. doi: 10.1073/pnas.62.3.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matsubara K. Properties of sex factor and related episomes isolated from purified Escherichia coli zygote cells. J Mol Biol. 1968 Nov 28;38(1):89–108. doi: 10.1016/0022-2836(68)90130-7. [DOI] [PubMed] [Google Scholar]
- Negrotti T., de Zwaig R. N. Interactions between Colicinogenic Factor B and F-Type Factors: Isolation of a Mutant F-lac Factor Insensitive to the Effect of Colicinogenic Factor B. Genetics. 1972 Feb;70(2):205–213. doi: 10.1093/genetics/70.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REVEL H. R. SYNTHESIS OF BETA-D-GALACTOSIDASE AFTER F-DUCTION OF LAC+GENES INTO ESCHERICHIA COLI. J Mol Biol. 1965 Jan;11:23–34. doi: 10.1016/s0022-2836(65)80168-1. [DOI] [PubMed] [Google Scholar]
- SCAIFE J., GROSS J. D. Inhibition of multiplication of an Flac factor in Hfr cells of Escherichia coli K-12. Biochem Biophys Res Commun. 1962 May 11;7:403–407. doi: 10.1016/0006-291x(62)90324-8. [DOI] [PubMed] [Google Scholar]
- Sharp P. A., Hsu M. T., Otsubo E., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972 Nov 14;71(2):471–497. doi: 10.1016/0022-2836(72)90363-4. [DOI] [PubMed] [Google Scholar]
- Sheehy R. J., Orr C., Curtiss R., 3rd Molecular studies on entry exclusion in Escherichia coli minicells. J Bacteriol. 1972 Nov;112(2):861–869. doi: 10.1128/jb.112.2.861-869.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vapnek D., Rupp W. D. Asymmetric segregation of the complementary sex-factor DNA strands during conjugation in Escherichia coli. J Mol Biol. 1970 Nov 14;53(3):287–303. doi: 10.1016/0022-2836(70)90066-5. [DOI] [PubMed] [Google Scholar]
- Willetts N. S. Characterization of the F transfer cistron, traL. Genet Res. 1973 Apr;21(2):205–213. doi: 10.1017/s0016672300013379. [DOI] [PubMed] [Google Scholar]
- Willetts N. S., Finnegan D. J. Characteristics of E. coli K12 strains carrying both an F prime and an R factor. Genet Res. 1970 Aug;16(1):113–122. doi: 10.1017/s0016672300002329. [DOI] [PubMed] [Google Scholar]
- Willetts N. S. Location of the origin of transfer of the sex factor F. J Bacteriol. 1972 Nov;112(2):773–778. doi: 10.1128/jb.112.2.773-778.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N., Achtman M. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation. J Bacteriol. 1972 Jun;110(3):843–851. doi: 10.1128/jb.110.3.843-851.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Willetts N., Maule J. Surface exclusion by ColV-K94. Genet Res. 1973 Jun;21(3):297–299. doi: 10.1017/s0016672300013495. [DOI] [PubMed] [Google Scholar]