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. 1971 Jul;107(1):121–129. doi: 10.1128/jb.107.1.121-129.1971

Effect of 2-Deoxyglucose on Cell Wall Formation in Saccharomyces cerevisiae and Its Relation to Cell Growth Inhibition

P Biely 1, Z Krátký 1, J Kovařík 1, Š Bauer 1
PMCID: PMC246894  PMID: 5563864

Abstract

The growth inhibition and the lysis of Saccharomyces cerevisiae caused by 2-deoxy-d-glucose (2-DG) were shown to be a consequence of unbalanced cellular growth and division. The lysis, but not the repression of growth and osmotic fragility of cells, could be suppressed by the addition of mannitol as an osmotic stabilizer. This result, as well as the morphological changes observed in the cells and changes in the chemical composition of the cell walls, showed that S. cerevisiae grown in the presence of 2-DG formed weakened cell walls responsible for the osmotic fragility. Evidence is presented for the first time demonstrating the incorporation of 2-DG into yeast cell wall material. Other data suggest that the inhibition of yeast growth by 2-DG results from an interference of phosphorylated metabolites of 2-DG with metabolic processes of glucose and mannose involved in the synthesis of structural cell wall polysaccharides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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