Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Jul;107(1):251–258. doi: 10.1128/jb.107.1.251-258.1971

Effect of Shift-Down and Growth Inhibition on Phospholipid Metabolism of Escherichia coli

J P G Ballesta a,1, M Schaechter a
PMCID: PMC246912  PMID: 4327511

Abstract

The metabolism of phospholipids of Escherichia coli was studied under conditions which inhibit various metabolic processes. Phospholipid synthesis and turnover were not inhibited by growth-inhibitory amounts of various antibiotics. Turnover of phosphatidylglycerol (PG) was inhibited by small amounts of dinitrophenol and by anaerobiosis. Turnover of phosphatidylethanolamine (PE), which is not detected in control cultures, was demonstrated under conditions of incipient lysis. When cells were shifted down from a rich to a poor medium, PE synthesis was inhibited, and incorporation of glycerol into the distal position of PG was stimulated. Under these conditions, turnover of the phosphate and the acylated glycerol moieties of PG was inhibited. Increased synthesis of PE was detected when filamentous cells were induced to make septa. The results indicate that PE synthesis is related to growth and cell division, whereas PG metabolism is related to other cell processes.

Full text

PDF
251

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chang Y. Y., Kennedy E. P. Biosynthesis of phosphatidyl glycerophosphate in Escherichia coli. J Lipid Res. 1967 Sep;8(5):447–455. [PubMed] [Google Scholar]
  3. Chang Y. Y., Kennedy E. P. Pathways for the synthesis of glycerophosphatides in Escherichia coli. J Biol Chem. 1967 Feb 10;242(3):516–519. [PubMed] [Google Scholar]
  4. Cronan J. E., Jr Phospholipid alterations during growth of Escherichia coli. J Bacteriol. 1968 Jun;95(6):2054–2061. doi: 10.1128/jb.95.6.2054-2061.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAWSON R. M. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples. Biochem J. 1960 Apr;75:45–53. doi: 10.1042/bj0750045. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dankert M., Wright A., Kelley W. S., Robbins P. W. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis. Arch Biochem Biophys. 1966 Sep 26;116(1):425–435. doi: 10.1016/0003-9861(66)90049-x. [DOI] [PubMed] [Google Scholar]
  7. Fox C. F. A lipid requirement for induction of lactose transport in Escherichia coli. Proc Natl Acad Sci U S A. 1969 Jul;63(3):850–855. doi: 10.1073/pnas.63.3.850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Furrow M. H., Pizer L. I. Phospholipid synthesis in Escherichia coli infected with T4 bacteriophages. J Virol. 1968 Jun;2(6):594–605. doi: 10.1128/jvi.2.6.594-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gale E. F. Effect of morphine derivatives on lipid metabolism in Staphylococcus aureus. Mol Pharmacol. 1970 Mar;6(2):134–145. [PubMed] [Google Scholar]
  10. HAYASHI S., KOCH J. P., LIN E. C. ACTIVE TRANSPORT OF L-ALPHA-GLYCEROPHOSPHATE IN ESCHERICHIA COLI. J Biol Chem. 1964 Sep;239:3098–3105. [PubMed] [Google Scholar]
  11. HIPP N. J., BASCH J. J., GORDON W. G. Amino acid composition of alpha1-, alpha2-, and alpha3-caseins. Arch Biochem Biophys. 1961 Jul;94:35–37. doi: 10.1016/0003-9861(61)90007-8. [DOI] [PubMed] [Google Scholar]
  12. KJELDGAARD N. O., MAALOE O., SCHAECHTER M. The transition between different physiological states during balanced growth of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):607–616. doi: 10.1099/00221287-19-3-607. [DOI] [PubMed] [Google Scholar]
  13. Kanemasa Y., Akamatsu Y., Nojima S. Composition and turnover of the phospholipids in Escherichia coli. Biochim Biophys Acta. 1967 Oct 2;144(2):382–390. [PubMed] [Google Scholar]
  14. McQUILLEN K., ROBERTS R. B. The utilization of acetate for synthesis in Escherichia coli. J Biol Chem. 1954 Mar;207(1):81–95. [PubMed] [Google Scholar]
  15. Milner L. S., Kaback H. R. The role of phosphatidylglycerol in the vectorial phosphorylation of sugar by isolated bacterial membrane preparations. Proc Natl Acad Sci U S A. 1970 Mar;65(3):683–690. doi: 10.1073/pnas.65.3.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Okuyama H. Phospholipid metabolism in Escherichia coli after a shift in temperature. Biochim Biophys Acta. 1969 Jan 21;176(1):125–134. [PubMed] [Google Scholar]
  17. Peterson R. H., Buller C. S. Phospholipid metabolism in T4 bacteriophage infected Escherichia coli K-12 (lambda). J Virol. 1969 May;3(5):463–468. doi: 10.1128/jvi.3.5.463-468.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Randle C. L., Albro P. W., Dittmer J. C. The phosphoglyceride composition of Gram-negative bacteria and the changes in composition during growth. Biochim Biophys Acta. 1969;187(2):214–220. doi: 10.1016/0005-2760(69)90030-7. [DOI] [PubMed] [Google Scholar]
  19. Rothfield L., Finkelstein A. Membrane biochemistry. Annu Rev Biochem. 1968;37:463–496. doi: 10.1146/annurev.bi.37.070168.002335. [DOI] [PubMed] [Google Scholar]
  20. Sastry P. S., Hokin L. E. Studies on the role of phospholipids in phagocytosis. J Biol Chem. 1966 Jul 25;241(14):3354–3361. [PubMed] [Google Scholar]
  21. Sokawa Y., Nakao E., Kaziro Y. On the nature of the control by RC gene in e. coli: amino acid-dependent control of lipid synthesis. Biochem Biophys Res Commun. 1968 Oct 10;33(1):108–112. doi: 10.1016/0006-291x(68)90263-5. [DOI] [PubMed] [Google Scholar]
  22. Stárka J., Moravová J. Phospholipids and cellular division of Escherichia coli. J Gen Microbiol. 1970 Feb;60(2):251–257. doi: 10.1099/00221287-60-2-251. [DOI] [PubMed] [Google Scholar]
  23. Vandor S. L., Richardson K. E. Incorporation of ethanolamine-1,2-14C into plant microsomal phospholipids. Can J Biochem. 1968 Oct;46(10):1309–1315. doi: 10.1139/o68-196. [DOI] [PubMed] [Google Scholar]
  24. Walker J. R., Pardee A. B. Conditional mutations involving septum formation in Escherichia coli. J Bacteriol. 1967 Jan;93(1):107–114. doi: 10.1128/jb.93.1.107-114.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. White D. C., Tucker A. N. Phospholipid metabolism during bacterial growth. J Lipid Res. 1969 Mar;10(2):220–233. [PubMed] [Google Scholar]
  26. White D. C., Tucker A. N. Phospholipid metabolism during changes in the proportions of membrane-bound respiratory pigments in Haemophilus parainfluenzae. J Bacteriol. 1969 Jan;97(1):199–209. doi: 10.1128/jb.97.1.199-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES