Abstract
Fragments of exosporium, isolated from dormant spores of Bacillus cereus, were disintegrated by treatment with sodium dodecyl sulfate (SDS) or with phenol and acetic acid. After centrifugation of each preparation, proteins in the supernatant fractions were resolved by disc gel electrophoresis into either two or eight bands, respectively. The SDS-solubilized fraction contained spheroidal particles 11 to 44 nm in diameter. When centrifuged until clear, this fraction after dialysis still gave rise to crystal-like sheets which had the same lattice symmetry and major chemical components (protein, lipid, and carbohydrate) as fragments of the native exosporium.
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Selected References
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- BRAKKE M. K. Photometric scanning of centrifuged density gradient columns. Anal Biochem. 1963 Apr;5:271–283. doi: 10.1016/0003-2697(63)90079-4. [DOI] [PubMed] [Google Scholar]
- Buckmire F. L., Murray R. G. Studies on the cell wall of Spirillum serpens. 1. Isolation and partial purification of the outermost cell wall layer. Can J Microbiol. 1970 Oct;16(10):1011–1022. doi: 10.1139/m70-171. [DOI] [PubMed] [Google Scholar]
- Butler T. F., Smith G. L., Grula E. A. Bacterial cell membranes. I. Reaggregation of membrane subunits from Micrococcus lysodeikticus. Can J Microbiol. 1967 Nov;13(11):1471–1479. doi: 10.1139/m67-195. [DOI] [PubMed] [Google Scholar]
- Engelman D. M., Morowitz H. J. Characterization of the plasma membrane of Mycoplasma laidlawii. IV. Structure and composition of membrane and aggregated components. Biochim Biophys Acta. 1968 Apr 29;150(3):385–396. doi: 10.1016/0005-2736(68)90137-5. [DOI] [PubMed] [Google Scholar]
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- GERHARDT P., RIBI E. ULTRASTRUCTURE OF THE EXOSPORIUM ENVELOPING SPORES OF BACILLUS CEREUS. J Bacteriol. 1964 Dec;88:1774–1789. doi: 10.1128/jb.88.6.1774-1789.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerhardt P. Cytology of Bacillus anthracis. Fed Proc. 1967 Sep;26(5):1504–1517. [PubMed] [Google Scholar]
- Green D. E., Allmann D. W., Bachmann E., Baum H., Kopaczyk K., Korman E. F., Lipton S., MacLennan D. H., McConnell D. G., Perdue J. F. Formation of membranes by repeating units. Arch Biochem Biophys. 1967 Mar;119(1):312–335. doi: 10.1016/0003-9861(67)90461-4. [DOI] [PubMed] [Google Scholar]
- Hiragi Y., Iijima K., Kadota H. Hexagonal single crystal pattern from the spore coat of Bacillus subtilis. Nature. 1967 Jul 8;215(5097):154–155. doi: 10.1038/215154a0. [DOI] [PubMed] [Google Scholar]
- Holt S. C., Leadbetter E. R. Comparative ultrastructure of selected aerobic spore-forming bacteria: a freeze-etching study. Bacteriol Rev. 1969 Jun;33(2):346–378. doi: 10.1128/br.33.2.346-378.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kushner D. J. Self-assembly of biological structures. Bacteriol Rev. 1969 Jun;33(2):302–345. doi: 10.1128/br.33.2.302-345.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Matz L. L., Beaman T. C., Gerhardt P. Chemical composition of exosporium from spores of Bacillus cereus. J Bacteriol. 1970 Jan;101(1):196–201. doi: 10.1128/jb.101.1.196-201.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mirsky R. Membrane proteins from Bacillus megaterium KM. Biochemistry. 1969 Mar;8(3):1164–1169. doi: 10.1021/bi00831a050. [DOI] [PubMed] [Google Scholar]
- Nermut M. V., Murray R. G. Ultrastructure of the cell wall of Bacillus polymyxa. J Bacteriol. 1967 Jun;93(6):1949–1965. doi: 10.1128/jb.93.6.1949-1965.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Razin S., Morowitz H. J., Terry T. M. Membrane subunits of Mycoplasma laidlawii and their assembly to membranelike structures. Proc Natl Acad Sci U S A. 1965 Jul;54(1):219–225. doi: 10.1073/pnas.54.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rottem S., Stein O., Razin S. Reassembly of Mycoplasma membranes disaggregated by detergents. Arch Biochem Biophys. 1968 Apr;125(1):46–56. doi: 10.1016/0003-9861(68)90637-1. [DOI] [PubMed] [Google Scholar]
- TOENNIES G., KOLB J. J. CARBOHYDRATE ANALYSIS OF BACTERIAL SUBSTANCES BY A NEW ANTHRONE PROCEDURE. Anal Biochem. 1964 May;8:54–69. doi: 10.1016/0003-2697(64)90168-x. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]