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. 1971 Aug;107(2):401–405. doi: 10.1128/jb.107.2.401-405.1971

Inactivation of 1,6-Diphosphatase by Glucose in Yeast

Carlos Gancedo 1
PMCID: PMC246939  PMID: 4329729

Abstract

Fructose-1,6-diphosphatase was derepressed in Saccharomyces cerevisiae by incubation in media containing non-sugar carbon sources. Addition of glucose to a derepressed culture led to a rapid loss of the measurable activity of the enzyme. Fructose and mannose also produced inactivation, but 2-deoxyglucose was ineffective. Experiments with cycloheximide indicated that the inactivation does not require protein synthesis. It was also shown that the process is not energy-dependent. The reappearance of the enzyme was dependent on an energy source and was prevented by cycloheximide. These results suggest that fructose diphosphatase inactivation is irreversible and that reappearance of enzyme activity implies de novo synthesis. Screening of different genera of yeasts has shown that the inactivation of fructose diphosphatase is a relatively widespread phenomenon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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