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. 1971 Aug;107(2):505–512. doi: 10.1128/jb.107.2.505-512.1971

Deoxyribonucleic Acid Transferred from Ultraviolet-Irradiated Excision-Defective Hfr Cells of Escherichia coli K-12

B M Wilkins 1, S E Hollom 2, W D Rupp 3
PMCID: PMC246953  PMID: 4939765

Abstract

Deoxyribonucleic acid (DNA) transfer from 3H-thymine-labeled Hfr cells has been measured by determining the amount of radioactivity remaining after selective lysis of the donor cells in the mating mixture. DNA transfer was less effectively reduced by ultraviolet irradiation of excision-defective Hfr cells than was the yield of recombinants. The buoyant density of DNA transferred from unirradiated and irradiated Hfr cells was equivalent to that of double-stranded DNA. Mating-dependent DNA synthesis in the recipient has been measured by mating Hfr cells deficient in thymidine kinase with irradiated thymine-requiring F− cells in the presence of 3H-thymine. The extent of such DNA synthesis approximated the amount of DNA transferred from unirradiated donors. Neither DNA transfer nor mating-dependent DNA synthesis could be reliably measured when both parents were irradiated. It is proposed that transferred Hfr DNA is replicated in the recipient and that this replication still occurs when the Hfr DNA contains dimers.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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