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. 1971 Aug;107(2):513–518. doi: 10.1128/jb.107.2.513-518.1971

Effect of Pleiotropic Carbohydrate Mutations (ctr) on Tryptophan Catabolism1

Rolf Dahl a, Richard J Wang a,2, M L Morse a
PMCID: PMC246954  PMID: 4329731

Abstract

The pleiotropic ctr mutation has been shown to affect tryptophan uptake and tryptophanase formation. Genetic reversions are of two types: (i) complete, restoring to wild type, located at 46 to 47 min; (ii) partial, restoring only tryptophanase synthesis, located at 73 min. In some strains the effect of ctr mutations could be reversed by cyclic adenosine 3′,5′-monophosphate (cAMP) plus tryptophan. A mutant producing tryptophanase constitutively was suppressed by a ctr mutation. Production of tryptophanase in this suppressed strain was not restored by the addition of cAMP, but required cAMP plus tryptophan.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURROUS S. E., DEMOSS R. D. STUDIES ON TRYPTOPHAN PERMEASE IN ESCHERICHIA COLI. Biochim Biophys Acta. 1963 Aug 6;73:623–637. doi: 10.1016/0006-3002(63)90332-9. [DOI] [PubMed] [Google Scholar]
  2. Berman M., Zwaig N., Lin E. C. Suppression of a pleiotropic mutant affecting glycerol dissimilation. Biochem Biophys Res Commun. 1970 Jan 23;38(2):272–278. doi: 10.1016/0006-291x(70)90708-4. [DOI] [PubMed] [Google Scholar]
  3. Brown K. D. Formation of aromatic amino acid pools in Escherichia coli K-12. J Bacteriol. 1970 Oct;104(1):177–188. doi: 10.1128/jb.104.1.177-188.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eggertsson G., Adelberg E. A. Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics. 1965 Aug;52(2):319–340. doi: 10.1093/genetics/52.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KESSLER D. P., RICKENBERG H. V. A NEW METHOD FOR THE SELECTION OF MUTANTS OF ESCHERICHIA COLI FORMING BETA-GALACTOSIDASE CONSTITUTIVELY. Biochim Biophys Acta. 1964 Sep 4;90:609–610. doi: 10.1016/0304-4165(64)90241-7. [DOI] [PubMed] [Google Scholar]
  6. Levinthal M. Biochemical studies of melibiose metabolism in wild type and mel mutant strains of Salmonella typhimurium. J Bacteriol. 1971 Mar;105(3):1047–1052. doi: 10.1128/jb.105.3.1047-1052.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MORSE M. L., ALIRE M. L. An agar medium indicating acid production. J Bacteriol. 1958 Sep;76(3):270–271. doi: 10.1128/jb.76.3.270-271.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PARDEE A. B., PRESTIDGE L. S. The initial kinetics of enzyme induction. Biochim Biophys Acta. 1961 Apr 29;49:77–88. doi: 10.1016/0006-3002(61)90871-x. [DOI] [PubMed] [Google Scholar]
  9. PITTARD J. EFFECT OF INTEGRATED SEX FACTOR ON TRANSDUCTION OF CHROMOSOMAL GENES IN ESCHERICHIA COLI. J Bacteriol. 1965 Mar;89:680–686. doi: 10.1128/jb.89.3.680-686.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pastan I., Perlman R. L. Repression of beta-galactosidase synthesis by glucose in phosphotransferase mutants of Escherichia coli. Repression in the absence of glucose phosphorylation. J Biol Chem. 1969 Nov 10;244(21):5836–5842. [PubMed] [Google Scholar]
  11. Pastan I., Perlman R. L. Stimulation of tryptophanase synthesis in Escherichia coli by cyclic 3',5'-adenosine monophosphate. J Biol Chem. 1969 Apr 25;244(8):2226–2232. [PubMed] [Google Scholar]
  12. Pastan I., Perlman R. Cyclic adenosine monophosphate in bacteria. Science. 1970 Jul 24;169(3943):339–344. doi: 10.1126/science.169.3943.339. [DOI] [PubMed] [Google Scholar]
  13. Rothman J. L. Transduction studies on the relation between prophage and host chromosome. J Mol Biol. 1965 Jul;12(3):892–912. doi: 10.1016/s0022-2836(65)80336-9. [DOI] [PubMed] [Google Scholar]
  14. Schwartz D. O., Beckwith J. R. Mutagens which cause deletions in Escherichia coli. Genetics. 1969 Feb;61(2):371–376. doi: 10.1093/genetics/61.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wang R. J., Morse H. G., Morse M. L. Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations. J Bacteriol. 1970 Dec;104(3):1318–1324. doi: 10.1128/jb.104.3.1318-1324.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wang R. J., Morse H. G., Morse M. L. Carbohydrate accumulation and metabolism in Escherichia coli: the close linkage and chromosomal location of ctr mutations. J Bacteriol. 1969 May;98(2):605–610. doi: 10.1128/jb.98.2.605-610.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wang R. J., Morse M. L. Carbohydrate accumulation and metabolism in Escherichia coli. I. Description of pleiotropic mutants. J Mol Biol. 1968 Feb 28;32(1):59–66. doi: 10.1016/0022-2836(68)90145-9. [DOI] [PubMed] [Google Scholar]

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