Abstract
A mutant of Saccharomyces cerevisiae deficient in phosphoglucoisomerase (EC 5.3.1.9) is described. It does not grow on glucose or sucrose but does grow on galactose or maltose. Addition of glucose to cultures growing on fructose, mannose, or acetate arrests further growth without altering viability; removal of glucose permits resumption of growth. Glucose causes accumulation of nearly 30 μmoles of glucose-6-phosphate per g (wet weight) of cells and suppresses synthesis of ribonucleic acid. Inhibition of growth by glucose does not appear to be due to a loss of adenosine triphosphate or inorganic orthophosphate. The mutant, however, utilizes glucose-6-phosphate produced intracellularly. Release of carbon dioxide from specifically labeled glucose suggests a C-l preferential cleavage. The kinetics of glucose-6-phosphate accumulation during glucose utilization in the mutant is not consistent with the notion that the utilization of glucose is controlled by glucose-6-phosphate.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bernheim N. J., Dobrogosz W. J. Amino sugar sensitivity in Escherichia coli mutants unable to grow on N-acetylglucosamine. J Bacteriol. 1970 Feb;101(2):384–391. doi: 10.1128/jb.101.2.384-391.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Böck A., Neidhardt F. C. Properties of a Mutant of Escherichia coli with a Temperature-sensitive Fructose-1,6-Diphosphate Aldolase. J Bacteriol. 1966 Aug;92(2):470–476. doi: 10.1128/jb.92.2.470-476.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CHANCE B. Energy-linked cytochrome oxidation in mitochondria. Nature. 1961 Mar 4;189:719–725. doi: 10.1038/189719b0. [DOI] [PubMed] [Google Scholar]
- Cozzarelli N. R., Koch J. P., Hayashi S., Lin E. C. Growth stasis by accumulated L-alpha-glycerophosphate in Escherichia coli. J Bacteriol. 1965 Nov;90(5):1325–1329. doi: 10.1128/jb.90.5.1325-1329.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE LA FUENTE G., SOLS A. Transport of sugars in yeasts. II. Mechanisms of utilization of disaccharides and related glycosides. Biochim Biophys Acta. 1962 Jan 1;56:49–62. doi: 10.1016/0006-3002(62)90526-7. [DOI] [PubMed] [Google Scholar]
- ESTABROOK R. W., MAITRA P. K. A fluorimetric method for the quantitative microanalysis of adenine and pyridine nucleotides. Anal Biochem. 1962 May;3:369–382. doi: 10.1016/0003-2697(62)90065-9. [DOI] [PubMed] [Google Scholar]
- FRAENKEL D., OSBORN M. J., HORECKER B. L., SMITH S. M. Metabolism and cell wall structure of a mutant of Salmonella typhimurium deficient in phosphoglucose isomerase. Biochem Biophys Res Commun. 1963 Jun 20;11:423–428. doi: 10.1016/0006-291x(63)90086-x. [DOI] [PubMed] [Google Scholar]
- Fraenkel D. G., Levisohn S. R. Glucose and gluconate metabolism in an Escherichia coli mutant lacking phosphoglucose isomerase. J Bacteriol. 1967 May;93(5):1571–1578. doi: 10.1128/jb.93.5.1571-1578.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraenkel D. G. The accumulation of glucose 6-phosphate from glucose and its effect in an Escherichia coli mutant lacking phosphoglucose isomerase and glucose 6-phosphate dehydrogenase. J Biol Chem. 1968 Dec 25;243(24):6451–6457. [PubMed] [Google Scholar]
- Görts C. P. Effect of glucose on the activity and the kinetics of the maltose-uptake system and of alpha-glucosidase in Saccharomyces cerevisiae. Biochim Biophys Acta. 1969 Jul 30;184(2):299–305. doi: 10.1016/0304-4165(69)90032-4. [DOI] [PubMed] [Google Scholar]
- HATTMAN S. THE FUNCTIONING OF T-EVEN PHAGES WITH UNGLUCOSYLATED DNA IN RESTRICTING ESCHERICHIA COLI HOST CELLS. Virology. 1964 Nov;24:333–348. doi: 10.1016/0042-6822(64)90171-0. [DOI] [PubMed] [Google Scholar]
- HEREDIA C. F., DELAFUENTE G., SOLS A. METABOLIC STUDIES WITH 2-DEOXYHEXOSES. I. MECHANISMS OF INHIBITION OF GROWTH AND FERMENTATION IN BAKER'S YEAST. Biochim Biophys Acta. 1964 May 11;86:216–223. doi: 10.1016/0304-4165(64)90045-5. [DOI] [PubMed] [Google Scholar]
- LEDERBERG J., LEDERBERG E. M. Replica plating and indirect selection of bacterial mutants. J Bacteriol. 1952 Mar;63(3):399–406. doi: 10.1128/jb.63.3.399-406.1952. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maitra P. K. A glucokinase from Saccharomyces cerevisiae. J Biol Chem. 1970 May 10;245(9):2423–2431. [PubMed] [Google Scholar]
- Maitra P. K., Lobo Z. A kinetic study of glycolytic enzyme synthesis in yeast. J Biol Chem. 1971 Jan 25;246(2):475–488. [PubMed] [Google Scholar]
- Maitra P. K., Lobo Z. Control of glycolytic enzyme synthesis in yeast by products of the hexokinase reaction. J Biol Chem. 1971 Jan 25;246(2):489–499. [PubMed] [Google Scholar]
- Megnet R. Effect of 2-deoxyglucose on Schizosaccharomyces pombe. J Bacteriol. 1965 Oct;90(4):1032–1035. doi: 10.1128/jb.90.4.1032-1035.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROBERTSON J. J., HALVORSON H. O. The components of maltozymase in yeast, and their behavior during deadaptation. J Bacteriol. 1957 Feb;73(2):186–198. doi: 10.1128/jb.73.2.186-198.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothman L. B., Cabib E. Regulation of glycogen synthesis in the intact yeast cell. Biochemistry. 1969 Aug;8(8):3332–3341. doi: 10.1021/bi00836a030. [DOI] [PubMed] [Google Scholar]
- Yarmolinsky M. B., Wiesmeyer H., Kalckar H. M., Jordan E. HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, II. GALACTOSE-INDUCED SENSITIVITY. Proc Natl Acad Sci U S A. 1959 Dec;45(12):1786–1791. doi: 10.1073/pnas.45.12.1786. [DOI] [PMC free article] [PubMed] [Google Scholar]