Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Sep;107(3):790–797. doi: 10.1128/jb.107.3.790-797.1971

Phospholipid Metabolism in the Absence of Net Phospholipid Synthesis in a Glycerol-Requiring Mutant of Bacillus subtilis

Thomas T Lillich 1, David C White 1
PMCID: PMC247002  PMID: 4999416

Abstract

A glycerol-requiring auxotroph of Bacillus subtilis showed no net synthesis of phospholipid when deprived of glycerol. Although there was no net synthesis of phospholipid, we found that: (i) fatty acids and 32P were slowly incorporated into phospholipid; (ii) in pulse-chase experiments, both 32P and 14C in the glycerol portion of the phospholipids were lost from phosphatidlyglycerol (PG) and lysylphosphatidylglycerol and accumulated in cardiolipin (CL); (iii) the proportions of the phospholipids in the membrane changed with a loss of PG and an accumulation of CL. The addition of glycerol to the glycerol-deprived cells resulted in a rapid incorporation of glycerol and restoration to the predeprivation metabolism and PG to CL ratio.

Full text

PDF
790

Images in this article

792Image
on p.792

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Ballesta J. P., Schaechter M. Effect of shift-down and growth inhibition on phospholipid metabolism of Escherichia coli. J Bacteriol. 1971 Jul;107(1):251–258. doi: 10.1128/jb.107.1.251-258.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop D. G., Rutberg L., Samuelsson B. The chemical composition of the cytoplasmic membrane of Bacillus subtilis. Eur J Biochem. 1967 Nov;2(4):448–453. doi: 10.1111/j.1432-1033.1967.tb00158.x. [DOI] [PubMed] [Google Scholar]
  4. Frerman F. E., White D. C. Membrane lipid changes during formation of a functional electron transport system in Staphylococcus aureus. J Bacteriol. 1967 Dec;94(6):1868–1874. doi: 10.1128/jb.94.6.1868-1874.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Henning U., Dennert G., Rehn K., Deppe G. Effects of oleate starvation in a fatty acid auxotroph of Escherichia coli K-12. J Bacteriol. 1969 May;98(2):784–796. doi: 10.1128/jb.98.2.784-796.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hsu C. C., Fox C. F. Induction of the lactose transport system in a lipid-synthesis-defective mutant of Escherichia coli. J Bacteriol. 1970 Aug;103(2):410–416. doi: 10.1128/jb.103.2.410-416.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Joyce G. H., White D. C. Effect of benzo(a) pyrene and piperonyl butoxide on formation of respiratory system, phospholipids, and carotenoids of Staphylococcus aureus. J Bacteriol. 1971 May;106(2):403–411. doi: 10.1128/jb.106.2.403-411.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mindich L. Induction of Staphylococcus aureus Lactose Permease in the Absence of Glycerolipid Synthesis. Proc Natl Acad Sci U S A. 1971 Feb;68(2):420–424. doi: 10.1073/pnas.68.2.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mindich L. Membrane synthesis in Bacillus subtilis. I. Isolation and properties of strains bearing mutations in glycerol metabolism. J Mol Biol. 1970 Apr 28;49(2):415–432. doi: 10.1016/0022-2836(70)90254-8. [DOI] [PubMed] [Google Scholar]
  10. Mindich L. Membrane synthesis in Bacillus subtilis. II. Integration of membrane proteins in the absence of lipid synthesis. J Mol Biol. 1970 Apr 28;49(2):433–439. doi: 10.1016/0022-2836(70)90255-x. [DOI] [PubMed] [Google Scholar]
  11. Mindich L. Pathway for oxidative dissimilation of glycerol in Bacillur subtilis. J Bacteriol. 1968 Aug;96(2):565–566. doi: 10.1128/jb.96.2.565-566.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ono Y., White D. C. Cardiolipin-specific phospholipase D activity in Haemophilus parainfluenzae. J Bacteriol. 1970 Jul;103(1):111–115. doi: 10.1128/jb.103.1.111-115.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ono Y., White D. C. Cardiolipin-specific phospholipase D of Haemophilus parainfluenzae. II. Characteristics and possible significance. J Bacteriol. 1970 Nov;104(2):712–718. doi: 10.1128/jb.104.2.712-718.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Short S. A., White D. C., Aleem M. I. Phospholipid metabolism in Ferrobacillus ferrooxidans. J Bacteriol. 1969 Jul;99(1):142–150. doi: 10.1128/jb.99.1.142-150.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Short S. A., White D. C. Metabolism of the glycosyl diglycerides and phosphatidylglucose of Staphylococcus aureus. J Bacteriol. 1970 Oct;104(1):126–132. doi: 10.1128/jb.104.1.126-132.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. VORBECK M. L., MARINETTI G. V. SEPARATION OF GLYCOSYL DIGLYCERIDES FROM PHOSPHATIDES USING SILICIC ACID COLUMN CHROMATOGRAPHY. J Lipid Res. 1965 Jan;6:3–6. [PubMed] [Google Scholar]
  17. White D. C., Frerman F. E. Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus. J Bacteriol. 1967 Dec;94(6):1854–1867. doi: 10.1128/jb.94.6.1854-1867.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. White D. C. Lipid composition of the electron transport membrane of Haemophilus parainfluenzae. J Bacteriol. 1968 Oct;96(4):1159–1170. doi: 10.1128/jb.96.4.1159-1170.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. White D. C., Tucker A. N. Phospholipid metabolism during bacterial growth. J Lipid Res. 1969 Mar;10(2):220–233. [PubMed] [Google Scholar]
  20. White D. C., Tucker A. N. Phospholipid metabolism during changes in the proportions of membrane-bound respiratory pigments in Haemophilus parainfluenzae. J Bacteriol. 1969 Jan;97(1):199–209. doi: 10.1128/jb.97.1.199-209.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilson G., Rose S. P., Fox C. F. The effect of membrane lipid unsaturation on glycoside transport. Biochem Biophys Res Commun. 1970 Feb 20;38(4):617–623. doi: 10.1016/0006-291x(70)90625-x. [DOI] [PubMed] [Google Scholar]
  22. Wuthier R. E. Two-dimensional chromatography on silica gel-loaded paper for the microanalysis of polar lipids. J Lipid Res. 1966 Jul;7(4):544–550. [PubMed] [Google Scholar]
  23. den Kamp J. A., Redai I., van Deenen L. L. Phospholipid composition of Bacillus subtilis. J Bacteriol. 1969 Jul;99(1):298–303. doi: 10.1128/jb.99.1.298-303.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES