Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Sep;107(3):815–823. doi: 10.1128/jb.107.3.815-823.1971

Protease Activities During the Course of Sporulation in Bacillus subtilis

Louise Prestidge 1, Vivian Gage 1, John Spizizen 1
PMCID: PMC247005  PMID: 4999417

Abstract

Three proteolytic enzymes have been isolated from sporulating cultures of Bacillus subtilis. These activities were, respectively, a protease inhibited by ethylenediaminetetraacetic acid (EDTA) but not phenylmethylsulfonyl fluoride (PMSF), a protease active on both protein and ester substrates, and an ester-active enzyme with low activity on proteins. The latter two enzymes were inhibited by PMSF but not by EDTA. The specific activity of each was determined both intra- and extra-cellularly during growth and sporulation in a single-defined medium. All three enzymes were shown to exhibit a rapid increase in specific activity at a time coinciding with the appearance of refractile bodies in cells.

Full text

PDF
815

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balassa G. Biochemical genetics of bacterial sporulation. I. Unidirectional pleiotropic interactions among genes controlling sporulation in Bacillus subtilis. Mol Gen Genet. 1969;104(1):73–103. [PubMed] [Google Scholar]
  2. Bernlohr R. W. Changes in amino acid permeation during sporulation. J Bacteriol. 1967 Mar;93(3):1031–1044. doi: 10.1128/jb.93.3.1031-1044.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyer H. W., Carlton B. C. Production of two proteolytic enzymes by a transformable strain of Bacillus subtilis. Arch Biochem Biophys. 1968 Nov;128(2):442–455. doi: 10.1016/0003-9861(68)90050-7. [DOI] [PubMed] [Google Scholar]
  4. DONNELLAN J. E., Jr, NAGS E. H., LEVINSON H. S. CHEMICALLY DEFINED, SYNTHETIC MEDIA FOR SPORULATION AND FOR GERMINATION AND GROWTH OF BACILLUS SUBTILIS. J Bacteriol. 1964 Feb;87:332–336. doi: 10.1128/jb.87.2.332-336.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Din F. U., Chaloupka J. Amino acid stimulation of proteinase synthesis in a sporogenous Bacillus megaterium KM. Biochem Biophys Res Commun. 1969 Oct 8;37(2):233–238. doi: 10.1016/0006-291x(69)90724-4. [DOI] [PubMed] [Google Scholar]
  6. FOSTER J. W. Morphogenesis in bacteria: some aspects of spore formation. Q Rev Biol. 1956 Jun;31(2):102–118. doi: 10.1086/401259. [DOI] [PubMed] [Google Scholar]
  7. GLAZER A. N., WELLNER D. Adsorption of proteins on 'Sephadex'. Nature. 1962 Jun 2;194:862–863. doi: 10.1038/194862a0. [DOI] [PubMed] [Google Scholar]
  8. HANSON R. S., SRINIVASAN V. R., HALVORSON H. O. Biochemistry of sporulation. I. Metabolism of acetate by vegetative and sporulating cells. J Bacteriol. 1963 Feb;85:451–460. doi: 10.1128/jb.85.2.451-460.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. JANSSEN F. W., LUND A. J., ANDERSON L. E. Colorimetric assay for dipicolinic acid in bacterial spores. Science. 1958 Jan 3;127(3288):26–27. doi: 10.1126/science.127.3288.26. [DOI] [PubMed] [Google Scholar]
  10. Kornberg A., Spudich J. A., Nelson D. L., Deutscher M. P. Origin of proteins in sporulation. Annu Rev Biochem. 1968;37:51–78. doi: 10.1146/annurev.bi.37.070168.000411. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Mandelstam J., Waites W. M. Sporulation in Bacillus subtilis. The role of exoprotease. Biochem J. 1968 Oct;109(5):793–801. doi: 10.1042/bj1090793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Millet J. Caractérisation de deux endopeptidases excrétées par B. subtilis Marburg au cours de la sporulation. Bull Soc Chim Biol (Paris) 1969 Jul 25;51(3):457–469. [PubMed] [Google Scholar]
  14. Millet J. Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation. J Appl Bacteriol. 1970 Mar;33(1):207–219. doi: 10.1111/j.1365-2672.1970.tb05245.x. [DOI] [PubMed] [Google Scholar]
  15. Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969 Mar;33(1):48–71. doi: 10.1128/br.33.1.48-71.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spudich J. A., Kornberg A. Biochemical studies of bacterial sporulation and germination. VI. Origin of spore core and coat proteins. J Biol Chem. 1968 Sep 10;243(17):4588–4599. [PubMed] [Google Scholar]
  18. Waites W. M. Sporulation in bacillus subtilis. Antigenic changes during spore formation. Biochem J. 1968 Oct;109(5):803–810. doi: 10.1042/bj1090803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Warren S. C. Sporulation in Bacillus subtilis. Biochemical changes. Biochem J. 1968 Oct;109(5):811–818. doi: 10.1042/bj1090811. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES