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. 1971 Oct;108(1):59–68. doi: 10.1128/jb.108.1.59-68.1971

Isolation, Mapping, and Characterization of Trehalaseless Mutants of Neurospora crassa

Alfred S Sussman a, M Kerry Garrett a, Malcolm Sargent a,1, Shih-An Yu a,2
PMCID: PMC247032  PMID: 5001211

Abstract

Mutant strains of Neurospora crassa that lack trehalase and are unable to grow on trehalose were isolated, and the gene (tre) was positioned on the right arm of linkage group I. Maltase and β-galactosidase activities are almost identical in tre− strains, whereas that of invertase was reduced by more than half and those of acid phosphatase and amylase were somewhat increased. Heterocaryons between standard and trehalaseless strains yield less than one-tenth the activity of the former. In addition, strains with duplications heterozygous for trehalase produce less than 1% of the activity of the standard strain. An inhibitor of trehalase has been found in tre− strains; its sensitivity to heat and proteolysis, and its nondialyzability suggest that this substance is a protein. The mig gene, which determines the rate of migration of trehalase on acrylamide gels, has been shown to be less than 1 map unit away from the tre gene.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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