Abstract
Cell-free extracts of Bacillus megaterium form β-cyanoalanine (β-CNA)-14C from Na14CN and l-cysteine, O-acetyl-l-serine or, to a lesser extent, l-serine. However, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of β-CNA from cysteine and cyanide. The formation of β-CNA is readily detected in extracts of cells grown in synthetic media with sulfate or l-djenkolic acid as sulfur sources; such cells also exhibit an increased ability to form cysteine when compared with cells grown on cysteine as the sulfur source. β-CNA formation could not be detected in extracts of cells grown on cysteine as the sulfur source. A 40-fold purification of the O-acetyl-serine sulfhydrylase resulted in the co-purification of the β-CNA-forming activity. The sulfhydrylase and the β-CNA-forming activity co-chromatographed on diethyl-aminoethyl cellulose and Sephadex G-100.
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