Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Oct;108(1):184–190. doi: 10.1128/jb.108.1.184-190.1971

Hyphal Wall Synthesis in Aspergillus nidulans: Effect of Protein Synthesis Inhibition and Osmotic Shock on Chitin Insertion and Morphogenesis

Dvorah Katz 1, R F Rosenberger 1
PMCID: PMC247049  PMID: 5001195

Abstract

Pulse-labeling with N-[acetyl-3H] glucosamine and radioautography were used to follow the sites of chitin incorporation in hyphae of an Aspergillus nidulans mutant blocked in amino sugar synthesis. Growing hyphae incorporated N-acetylglucosamine almost exclusively at the tip. Cycloheximide addition greatly increased the label in subapical regions of the hyphae and reduced that at the tip. This effect of cycloheximide was immediate, could be reversed by removing the inhibitor, and did not appear to be due to chitin turnover. A similar change from apical to subapical N-acetylglucosamine incorporation occurred after hyphae were subjected to an osmotic shock which did not inhibit protein synthesis. The two treatments induced morphogenetic changes in the hyphae which produced abnormally large numbers of branches and septa.

Full text

PDF
184

Images in this article

186Image
on p.186

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barratt R. W., Johnson G. B., Ogata W. N. Wild-type and mutant stocks of Aspergillus nidulans. Genetics. 1965 Jul;52(1):233–246. doi: 10.1093/genetics/52.1.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bartnicki-Garcia S. Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annu Rev Microbiol. 1968;22:87–108. doi: 10.1146/annurev.mi.22.100168.000511. [DOI] [PubMed] [Google Scholar]
  3. Bartnicki-Garcia S., Lippman E. Fungal morphogenesis: cell wall construction in Mucor rouxii. Science. 1969 Jul 18;165(3890):302–304. doi: 10.1126/science.165.3890.302. [DOI] [PubMed] [Google Scholar]
  4. Clutterbuck A. J. Synchronous nuclear division and septation in Aspergillus nidulans. J Gen Microbiol. 1970 Jan;60(1):133–135. doi: 10.1099/00221287-60-1-133. [DOI] [PubMed] [Google Scholar]
  5. Grove S. N., Bracker C. E. Protoplasmic organization of hyphal tips among fungi: vesicles and Spitzenkörper. J Bacteriol. 1970 Nov;104(2):989–1009. doi: 10.1128/jb.104.2.989-1009.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Haidle C. W., Storck R. Inhibition by cycloheximide of protein and RNA synthesis in Mucor rouxii. Biochem Biophys Res Commun. 1966 Jan 24;22(2):175–180. doi: 10.1016/0006-291x(66)90428-1. [DOI] [PubMed] [Google Scholar]
  7. Katz D., Rosenberger R. F. A mutation in Aspergillus nidulans producing hyphal walls which lack chitin. Biochim Biophys Acta. 1970 Jun;208(3):452–460. doi: 10.1016/0304-4165(70)90218-7. [DOI] [PubMed] [Google Scholar]
  8. Katz D., Rosenberger R. F. The utilisation of galactose by an Aspergillus nidulans mutant lacking galactose phosphate-UDP glucose transferase and its relation to cell wall synthesis. Arch Mikrobiol. 1970 Oct 21;74(1):41–51. doi: 10.1007/BF00408686. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Mahadevan P. R., Tatum E. L. Relationship of the major constituents of the Neurospora crassa cell wall to wild-type and colonial morphology. J Bacteriol. 1965 Oct;90(4):1073–1081. doi: 10.1128/jb.90.4.1073-1081.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marchant R., Smith D. G. A serological investigation of hyphal growth in Fusarium culmorum. Arch Mikrobiol. 1968;63(1):85–94. doi: 10.1007/BF00407067. [DOI] [PubMed] [Google Scholar]
  12. McClure W. K., Park D., Robinson P. M. Apical organization in the somatic hyphae of fungi. J Gen Microbiol. 1968 Feb;50(2):177–182. doi: 10.1099/00221287-50-2-177. [DOI] [PubMed] [Google Scholar]
  13. Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
  14. POTGIETER H. J., ALEXANDER M. POLYSACCHARIDE COMPONENTS OF NEUROSPORA CRASSA HYPHAL WALLS. Can J Microbiol. 1965 Feb;11:122–125. doi: 10.1139/m65-017. [DOI] [PubMed] [Google Scholar]
  15. Pooley H. M., Shockman G. D. Relationship between the location of autolysin, cell wall synthesis, and the development of resistance to cellular autolysis in Streptococcus faecalis after inhibition of protein synthesis. J Bacteriol. 1970 Aug;103(2):457–466. doi: 10.1128/jb.103.2.457-466.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rogers H. J. Bacterial growth and the cell envelope. Bacteriol Rev. 1970 Jun;34(2):194–214. doi: 10.1128/br.34.2.194-214.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SIEGEL M. R., SISLER H. D. SITE OF ACTION OF CYCLOHEXIMIDE IN CELLS OF SACCHAROMYCES PASTORIANUS. I. EFFECT OF THE ANTIBIOTIC ON CELLULAR METABOLISM. Biochim Biophys Acta. 1964 May 18;87:70–82. doi: 10.1016/0926-6550(64)90048-9. [DOI] [PubMed] [Google Scholar]
  18. Wiley W. R. Tryptophan transport in Neurospora crassa: a tryptophan-binding protein released by cold osmotic shock. J Bacteriol. 1970 Sep;103(3):656–662. doi: 10.1128/jb.103.3.656-662.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES