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. 1971 Oct;108(1):282–286. doi: 10.1128/jb.108.1.282-286.1971

Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks1

K Dimmitt a, M I Simon a
PMCID: PMC247063  PMID: 4107809

Abstract

A method for preparing bacterial flagellar hook structures is described. The method involves isolating intact flagella from a mutant which makes thermally labile flagellar filaments and heat-treating them to disaggregate the filament preferentially. The resulting hook preparation can be separated and purified by velocity and isopycnic centrifugation. The purified hooks sediment at a relative S value of 77. On acrylamide gel electrophoresis in sodium dodecyl sulfate, they show one major and a number of minor protein bands. The purified hooks can be used to immunize rabbits, and the resulting antiserum is hook-specific. These results support the notion that hooks are composed of a protein that differs from flagellin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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