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. 1971 Oct;108(1):451–458. doi: 10.1128/jb.108.1.451-458.1971

Cold Osmotic Shock in Saccharomyces cerevisiae

J W Patching 1, A H Rose 1
PMCID: PMC247085  PMID: 5001201

Abstract

Saccharomyces cerevisiae NCYC 366 is susceptible to cold osmotic shock. Exponentially growing cells from batch cultures grown in defined medium at 30 C, after being suspended in 0.8 m mannitol containing 10 mm ethylenedia-minetetraacetic acid and then resuspended in ice-cold 0.5 mm MgCl2, accumulated the nonmetabolizable solutes d-glucosamine-hydrochloride and 2-aminoisobutyrate at slower rates than unshocked cells; shocked cells retained their viability. Storage of unshocked batch-grown cells in buffer at 10 C led to an increase in ability to accumulate glucosamine, and further experiments were confined to cells grown in a chemostat under conditions of glucose limitation, thereby obviating the need for storing cells before use. A study was made of the effect of the different stages in the cold osmotic shock procedure, including the osmotic stress, the chelating agent, and the cold Mg2+-containing diluent, on viability and solute-accumulating ability. Growth of shocked cells in defined medium resembled that of unshocked cells; however, in malt extract-yeast extract-glucose-peptone medium, the shocked cells had a longer lag phase of growth and initially grew at a slower rate. Cold osmotic shock caused the release of low-molecular-weight compounds and about 6 to 8% of the cell protein. Neither the cell envelope enzymes, invertase, acid phosphatase and l-leucine-β-naphthylamidase, nor the cytoplasmic enzyme, alkaline phosphatase, were released when yeast cells were subjected to cold osmotic shock.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boer P., Steyn-Parvé E. P. Isolation and purification of an acid phosphatase from baker's yeast (Saccharomyces cerevisiae). Biochim Biophys Acta. 1966 Nov 15;128(2):400–402. doi: 10.1016/0926-6593(66)90189-5. [DOI] [PubMed] [Google Scholar]
  2. Brown C. M., Rose A. H. Effects of temperature on composition and cell volume of Candida utilis. J Bacteriol. 1969 Jan;97(1):261–270. doi: 10.1128/jb.97.1.261-272.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown C. M., Rose A. H. Fatty-acid composition of Candida utilis as affected by growth temperature and dissolved-oxygen tension. J Bacteriol. 1969 Aug;99(2):371–378. doi: 10.1128/jb.99.2.371-378.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diamond R. J., Rose A. H. Osmotic properties of spheroplasts from Saccharomyces cerevisiae grown at different temperatures. J Bacteriol. 1970 May;102(2):311–319. doi: 10.1128/jb.102.2.311-319.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Farrell J., Rose A. H. Cold shock in a mesophilic and a psychrophilic pseudomonad. J Gen Microbiol. 1968 Mar;50(3):429–439. doi: 10.1099/00221287-50-3-429. [DOI] [PubMed] [Google Scholar]
  6. GERHARDT P., JUDGE J. A. POROSITY OF ISOLATED CELL WALLS OF SACCHAROMYCES CEREVISIAE AND BACILLUS MEGATERIUM. J Bacteriol. 1964 Apr;87:945–951. doi: 10.1128/jb.87.4.945-951.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GOLDBARG J. A., RUTENBURG A. M. The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases. Cancer. 1958 Mar-Apr;11(2):283–291. doi: 10.1002/1097-0142(195803/04)11:2<283::aid-cncr2820110209>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  8. GORRILL R. H., McNEIL E. M. The effect of cold diluent on the viable count of Pseudomonas pyocyanea. J Gen Microbiol. 1960 Apr;22:437–442. doi: 10.1099/00221287-22-2-437. [DOI] [PubMed] [Google Scholar]
  9. Hegarty C. P., Weeks O. B. Sensitivity of Escherichia coli to Cold-Shock during the Logarithmic Growth Phase. J Bacteriol. 1940 May;39(5):475–484. doi: 10.1128/jb.39.5.475-484.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heppel L. A. Selective release of enzymes from bacteria. Science. 1967 Jun 16;156(3781):1451–1455. doi: 10.1126/science.156.3781.1451. [DOI] [PubMed] [Google Scholar]
  11. Kundig W., Kundig F. D., Anderson B., Roseman S. Restoration of active transport of glycosides in Escherichia coli by a component of a phosphotransferase system. J Biol Chem. 1966 Jul 10;241(13):3243–3246. [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Lampen J. O. External enzymes of yeast: their nature and formation. Antonie Van Leeuwenhoek. 1968;34(1):1–18. doi: 10.1007/BF02046409. [DOI] [PubMed] [Google Scholar]
  14. Longley R. P., Rose A. H., Knights B. A. Composition of the protoplast membrane from Saccharomyces cerevisiae. Biochem J. 1968 Jul;108(3):401–412. doi: 10.1042/bj1080401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MCLELLAN W. L., Jr, LAMPEN J. O. The acid phosphatase of yeast. Localization and secretion by protoplasts. Biochim Biophys Acta. 1963 Feb 12;67:324–326. doi: 10.1016/0006-3002(63)91832-8. [DOI] [PubMed] [Google Scholar]
  16. MEYNELL G. G. The effect of sudden chilling on Escherichia coli. J Gen Microbiol. 1958 Oct;19(2):380–389. doi: 10.1099/00221287-19-2-380. [DOI] [PubMed] [Google Scholar]
  17. MINARI O., ZILVERSMIT D. B. USE OF KCN FOR STABILIZATION OF COLOR IN DIRECT NESSLERIZATION OF KJELDAHL DIGESTS. Anal Biochem. 1963 Oct;6:320–327. doi: 10.1016/0003-2697(63)90156-8. [DOI] [PubMed] [Google Scholar]
  18. MacLennan D. G., Pirt S. J. Automatic control of dissolved oxygen concentration in stirred microbial cultures. J Gen Microbiol. 1966 Nov;45(2):289–302. doi: 10.1099/00221287-45-2-289. [DOI] [PubMed] [Google Scholar]
  19. NICKERSON W. J., ROSE A. H. Secretion of nicotinic acid by biotin-dependent yeasts. J Bacteriol. 1956 Sep;72(3):324–328. doi: 10.1128/jb.72.3.324-328.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Neu H. C., Chou J. Release of surface enzymes in Enterobacteriaceae by osmotic shock. J Bacteriol. 1967 Dec;94(6):1934–1945. doi: 10.1128/jb.94.6.1934-1945.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Neu H. C., Heppel L. A. On the surface localization of enzymes in E. coli. Biochem Biophys Res Commun. 1964 Oct 14;17(3):215–219. doi: 10.1016/0006-291x(64)90386-9. [DOI] [PubMed] [Google Scholar]
  22. Neumann N. P., Lampen J. O. Purification and properties of yeast invertase. Biochemistry. 1967 Feb;6(2):468–475. doi: 10.1021/bi00854a015. [DOI] [PubMed] [Google Scholar]
  23. Neumann N. P., Lampen J. O. The glycoprotein structure of yeast invertase. Biochemistry. 1969 Sep;8(9):3552–3556. doi: 10.1021/bi00837a010. [DOI] [PubMed] [Google Scholar]
  24. Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
  25. POSTGATE J. R., CRUMPTON J. E., HUNTER J. R. The measurement of bacterial viabilities by slide culture. J Gen Microbiol. 1961 Jan;24:15–24. doi: 10.1099/00221287-24-1-15. [DOI] [PubMed] [Google Scholar]
  26. Pardee A. B., Prestidge L. S., Whipple M. B., Dreyfuss J. A binding site for sulfate and its relation to sulfate transport into Salmonella typhimurium. J Biol Chem. 1966 Sep 10;241(17):3962–3969. [PubMed] [Google Scholar]
  27. SCHLENK F., DAINKO J. L. ACTION OF RIBONUCLEASE PREPARATIONS ON VIABLE YEAST CELLS AND SPHEROPLASTS. J Bacteriol. 1965 Feb;89:428–436. doi: 10.1128/jb.89.2.428-436.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. STRANGE R. E., DARK F. A. Effect of chilling on Aerobacter aerogenes in aqueous suspension. J Gen Microbiol. 1962 Dec;29:719–730. doi: 10.1099/00221287-29-4-719. [DOI] [PubMed] [Google Scholar]
  29. STRANGE R. E., NESS A. G. Effect of chilling on bacteria in aqueous suspension. Nature. 1963 Feb 23;197:819–819. doi: 10.1038/197819a0. [DOI] [PubMed] [Google Scholar]
  30. SUTTON D. D., LAMPEN J. O. Localization of sucrose and maltose fermenting systems in Saccharomyces cerevisiae. Biochim Biophys Acta. 1962 Jan 29;56:303–312. doi: 10.1016/0006-3002(62)90567-x. [DOI] [PubMed] [Google Scholar]
  31. Schlenk F., Dainko J. L. Effects of ribonuclease and spermine on yeast cells. Arch Biochem Biophys. 1966 Jan;113(1):127–133. doi: 10.1016/0003-9861(66)90165-2. [DOI] [PubMed] [Google Scholar]
  32. Sherman J. M., Albus W. R. Physiological Youth in Bacteria. J Bacteriol. 1923 Mar;8(2):127–139. doi: 10.1128/jb.8.2.127-139.1923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wiley W. R. Tryptophan transport in Neurospora crassa: a tryptophan-binding protein released by cold osmotic shock. J Bacteriol. 1970 Sep;103(3):656–662. doi: 10.1128/jb.103.3.656-662.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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