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. 1971 Oct;108(1):492–500. doi: 10.1128/jb.108.1.492-500.1971

Antimicrobial Actions of Hexachlorophene: Release of Cytoplasmic Materials1

H L Joswick a,2, Thomas R Corner a, J N Silvernale a,3, Philipp Gerhardt a
PMCID: PMC247090  PMID: 4330741

Abstract

Intracellular solutes were released from growing or resting cells of Bacillus megaterium as a consequence of hexachlorophene treatment. The effect was dose dependent, with the optimum at a concentration about sevenfold greater than the minimal lethal dose. The effects of pH and temperature on the leakage process also were inconsistent with the killing effects of the drug. The types of materials released appeared to be the same with or without hexachlorophene treatment. The released materials were small molecules which apparently derived from preexisting ribonucleic acid and protein, but not from deoxyribonucleic acid. Compared to the effects of other representative surface-active agents and other bis-phenols, hexachlorophene was superior in ability to cause leakage of intracellular materials. Different microorganisms varied in their susceptibility to hexachlorophene, with Pseudomonas aeruginosa and a paracolon isolate the most resistant of the vegetative cells examined. It was concluded that the release of intracellular solutes was an effect secondary to the lethal event and presumably arose from hexachlorophene-mediated stimulation of degradative enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANAND N., DAVIS B. D., ARMITAGE A. K. Uptake of streptomycin by Escherichia coli. Nature. 1960 Jan 2;185:23–24. doi: 10.1038/185023a0. [DOI] [PubMed] [Google Scholar]
  2. BRITT E. M., GERHARDT P. Bacterial permeability; total uptake of lysine by intact cells, protoplasts, and cell walls of Micrococcus lysodeikticus. J Bacteriol. 1958 Sep;76(3):288–293. doi: 10.1128/jb.76.3.288-293.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COREY R. R., SHIRK H. G. The influence of chemical structure on fungal activity. IV. The effect of bisphenolic-type compounds. Arch Biochem Biophys. 1955 May;56(1):196–203. doi: 10.1016/0003-9861(55)90348-9. [DOI] [PubMed] [Google Scholar]
  4. Corner T. R., Joswick H. L., Silvernale J. N., Gerhardt P. Antimicrobial actions of hexachlorophene: lysis and fixation of bacterial protoplasts. J Bacteriol. 1971 Oct;108(1):501–507. doi: 10.1128/jb.108.1.501-507.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DELAMATER E. D., BABCOCK K. L., MAZZANTI G. R. On the leakage of cellular material from Bacillus megaterium. J Bacteriol. 1959 Apr;77(4):513–514. doi: 10.1128/jb.77.4.513-514.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FLORESTANO H. J., BAHLER M. E. Antibacterial activity of a series of diphenylmethanes. J Am Pharm Assoc Am Pharm Assoc. 1953 Sep;42(9):576–578. doi: 10.1002/jps.3030420916. [DOI] [PubMed] [Google Scholar]
  7. GOULD B. S., BOSNIAK M. A., NEIDLEMAN S., GATT S. Effect of hexachlorophene and related bisphenolic compounds on the dehydrogenases and cytochrome system of Bacillus subtilis and Escherichia coli. Arch Biochem Biophys. 1953 Jun;44(2):284–297. doi: 10.1016/0003-9861(53)90046-0. [DOI] [PubMed] [Google Scholar]
  8. HUGO W. B., LONGWORTH A. R. EFFECT OF CHLORHEXIDINE DIACETATE ON "PROTOPLASTS" AND SPHEROPLASTS OF ESCHERICHIA COLI, PROTOPLASTS OF BACILLUS MEGATERIUM AND THE GRAM STAINING REACTION OF STAPHYLOCOCCUS AUREUS. J Pharm Pharmacol. 1964 Nov;16:751–758. doi: 10.1111/j.2042-7158.1964.tb07400.x. [DOI] [PubMed] [Google Scholar]
  9. Hugo W. B. The mode of action of antibacterial agents. J Appl Bacteriol. 1967 Apr;30(1):17–50. doi: 10.1111/j.1365-2672.1967.tb00273.x. [DOI] [PubMed] [Google Scholar]
  10. LAWRENCE C. A., ERLANDSON A. L., Jr A new inactivating medium for hexachlorophene (G-11). J Am Pharm Assoc Am Pharm Assoc. 1953 Jun;42(6):352–357. doi: 10.1002/jps.3030420612. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Marquis R. E. Osmotic sensitivity of bacterial protoplasts and the response of their limiting membrane to stretching. Arch Biochem Biophys. 1967 Feb;118(2):323–331. doi: 10.1016/0003-9861(67)90356-6. [DOI] [PubMed] [Google Scholar]
  13. McQUILLEN K. Bacterial protoplasts. I. Protein and nucleic acid metabolism in protoplasts of Bacillus megaterium. Biochim Biophys Acta. 1955 Jul;17(3):382–390. doi: 10.1016/0006-3002(55)90387-5. [DOI] [PubMed] [Google Scholar]
  14. Ordal E. J., Wilson J. L., Borg A. F. Studies on the Action of Wetting Agents on Microörganisms: I. The Effect of pH and Wetting Agents on the Germicidal Action of Phenolic Compounds. J Bacteriol. 1941 Jul;42(1):117–126. doi: 10.1128/jb.42.1.117-126.1941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROTH H., AMOS H., DAVIS B. D. Purine nucleotide excretion by Escherichia coli in the presence of streptomycin. Biochim Biophys Acta. 1960 Jan 29;37:398–405. doi: 10.1016/0006-3002(60)90495-9. [DOI] [PubMed] [Google Scholar]
  16. SALTON M. R. J. The adsorption of cetyltrimethylammonium bromide by bacteria, its action in releasing cellular constituents and its bactericidal effects. J Gen Microbiol. 1951 May;5(2):391–404. doi: 10.1099/00221287-5-2-391. [DOI] [PubMed] [Google Scholar]
  17. Scherrer R., Gerhardt P. Molecular sieving by the Bacillus megaterium cell wall and protoplast. J Bacteriol. 1971 Sep;107(3):718–735. doi: 10.1128/jb.107.3.718-735.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Silvernale J. N., Joswick H. L., Corner T. R., Gerhardt P. Antimicrobial actions of hexachlorophene: cytological manifestations. J Bacteriol. 1971 Oct;108(1):482–491. doi: 10.1128/jb.108.1.482-491.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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