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. 1971 Nov;108(2):690–694. doi: 10.1128/jb.108.2.690-694.1971

Biochemical Characterization of the ctr Mutants of Escherichia coli1

Helvise G Morse a, William K Penberthy a, M L Morse a
PMCID: PMC247127  PMID: 4942759

Abstract

A test procedure based on complementation in mixed extracts is described for the assay of heat-stable protein and enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system. The test was used to assay a collection of pleiotropic carbohydrate mutants of Escherichia coli (ctr mutants) and revertants of these mutants. All mutants were found to lack enzyme I of the phosphoenolpyruvate-dependent transferase system. Revertants of these mutants to complete wild phenotype regained enzyme I-forming ability. Reversion to partial wild type was not accompanied by restoration of enzyme I-forming ability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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