Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1971 Nov;108(2):751–756. doi: 10.1128/jb.108.2.751-756.1971

Oxidation of Nicotinic Acid by a Bacillus Species: Purification and Properties of Nicotinic Acid and 6-Hydroxynicotinic Acid Hydroxylases

Rona Hirschberg a,1, J C Ensign a
PMCID: PMC247135  PMID: 5128334

Abstract

The enzymes of a Bacillus species that hydroxylate nicotinic acid to 6-hydroxynicotinic acid and 6-hydroxynicotinic acid to 2,6-dihydroxynicotinic acid were purified and characterized. The purified enzymes contained approximately two molecules of flavine and eight molecules of iron per molecule of enzyme. The enzymes were large (molecular weight, 400,000 to 450,000) and appeared to consist of subunits.

Full text

PDF
751

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BEHRMAN E. J., STANIER R. Y. The bacterial oxidation of nicotinic acid. J Biol Chem. 1957 Oct;228(2):923–945. [PubMed] [Google Scholar]
  2. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  3. DOEG K. A., ZIEGLER D. M. Simplified methods for the estimation of iron in mitochondria and submitochondrial fractions. Arch Biochem Biophys. 1962 Apr;97:37–40. doi: 10.1016/0003-9861(62)90041-3. [DOI] [PubMed] [Google Scholar]
  4. ENSIGN J. C., RITTENBERG S. C. THE PATHWAY OF NICOTINIC ACID OXIDATION BY A BACILLUS SPECIES. J Biol Chem. 1964 Jul;239:2285–2291. [PubMed] [Google Scholar]
  5. Gaertner F. H., DeMoss J. A. Purification and characterization of a multienzyme complex in the tryptophan pathway of Neurospora crassa. J Biol Chem. 1969 May 25;244(10):2716–2725. [PubMed] [Google Scholar]
  6. HARARY I. Bacterial fermantation of nicotinic acid. II. Anaerobic reversible hydroxylation of nicotinic acid to 6-hydroxynicotinic acid. J Biol Chem. 1957 Aug;227(2):823–831. [PubMed] [Google Scholar]
  7. HARARY I. Bacterial fermentation of nicotinic acid. I. End products. J Biol Chem. 1957 Aug;227(2):815–822. [PubMed] [Google Scholar]
  8. HUGHES D. E. 6-Hydroxynicotinic acid as an intermediate in the oxidation of nicotinic acid by Pseudomonas fluorescens. Biochem J. 1955 Jun;60(2):303–310. doi: 10.1042/bj0600303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HUNT A. L. Purification of the nicotinic acid hydroxylase system of Pseudomonas fluorescens KB1. Biochem J. 1959 May;72(1):1–7. doi: 10.1042/bj0720001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirschberg R., Ensign J. C. Oxidation of nicotinic acid by a Bacillus species: source of oxygen atoms for the hydroxylation of nicotinic acid and 6-hydroxynicotinic acid. J Bacteriol. 1971 Nov;108(2):757–759. doi: 10.1128/jb.108.2.757-759.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hochstein L. I., Dalton B. P. The hydroxylation of nicotine: the origin of the hydroxyl oxygen. Biochem Biophys Res Commun. 1965 Dec 21;21(6):644–648. doi: 10.1016/0006-291x(65)90535-8. [DOI] [PubMed] [Google Scholar]
  12. Hochstein L. I., Dalton B. P. The purification and properties of nicotine oxidase. Biochim Biophys Acta. 1967 May 16;139(1):56–68. doi: 10.1016/0005-2744(67)90113-1. [DOI] [PubMed] [Google Scholar]
  13. Holcenberg J. S., Stadtman E. R. Nicotinic acid metabolism. 3. Purification and properties of a nicotinic acid hydroxylase. J Biol Chem. 1969 Mar 10;244(5):1194–1203. [PubMed] [Google Scholar]
  14. Holcenberg J. S., Tsai L. Nicotinic acid metabolism. IV. Ferredoxin-dependent reduction of 6-hydroxynicotinic acid to 6-oxo-1,4,5,6-tetrahydronicotinic acid. J Biol Chem. 1969 Mar 10;244(5):1204–1211. [PubMed] [Google Scholar]
  15. KOIKE M., REED L. J., CARROLL W. R. alpha-Keto acid dehydrogenation complexes. IV. Resolution and reconstitution of the Escherichia coli pyruvate dehydrogenation complex. J Biol Chem. 1963 Jan;238:30–39. [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. PASTAN I., TSAI L., STADTMAN E. R. NICOTINIC ACID METABOLISM. I. DISTRIBUTION OF ISOTOPE IN FERMENTATION PRODUCTS OF LABELLED NICOTINIC ACID. J Biol Chem. 1964 Mar;239:902–906. [PubMed] [Google Scholar]
  18. Tsai L., Pastan I., Stadtman E. R. Nicotinic acid metabolism. II. The isolation and characterization of intermediates in the fermentation of nicotinic acid. J Biol Chem. 1966 Apr 25;241(8):1807–1813. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES